Template:O2k-OpenSupportAlert2021

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

Apr 2021

Why is the fluorescence signal constantly at 10 V or at 0 V?

Follow these steps to localize and solve the problem:
- »Fluorescence signal is constantly at 10 V« and »0 V fluorescence signal«

Noisy fluorescence signal?

Follow these steps to localize and solve the problem:
- »Noisy fluorescence signal«

Do you know what to do when the fluorescence signal starts at a negative value?

A negative signal may be caused by wet Fluo-Sensors. For further information:
- »Negative fluorescence signal«

A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«

O2k-checklist: Get started with an O2k-experiment

The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
- » MiPNet20.04_O2k-checklist«
- » communicated by »Cecatto Cristiane« and »Schmitt Sabine«

Do you know how to prepare permeabilized muscle fibers?

The application of high-resolution respirometry in permeabilized muscle fibers provides a sensitive diagnostic tool for the analysis of mitochondrial (dys)function in small biopsy samples.
- »O2k-Videosupport: Fiber permeabilization«
- »Doerrier 2018 Methods Mol Biol«
- communicated by »Doerrier Carolina« and »Tindle-Solomon Lisa«

Do you know how to properly insert the ISS-tip into the O2k-chamber?

With the stirrer bar still rotating, insert the ISS-tip to the bottom of the O2k-Chamber, so that it points away from you.
- »MiPNet19.03 O2k-cleaning and ISS: Section 2«
- communicated by »Doerrier Carolina«, »Cecatto Cristiane« and »Komlodi Timea«

DatLab error messages: What do they mean and how to solve them?

Are you a beginner or need a refresher on using SUIT protocols with DatLab7.4?