From Bioblast
Description
Ruthenium red (synonym: ammoniated ruthenium oxychloride) inhibits the mitochondrial Ca2+ uniporter. However, in addition it has been shown to interact with and inhibit a large number of other proteins, including ion channels particularly of the Transient Receptor Potential Vanilloid (TRPV) family [1], Ca2+-ATPases, and, importantly, the voltage-dependent anion channel (VDAC) [2].
Abbreviation: RR
Reference: MiPNet03.02, Wikipedia
Application in HRR
- Rut: Ruthenium Red (technical grade; [(NH3)5RuORu(NH3)4ORu(NH3)5]Cl6); Sigma R 2751, 1 g; M = 786.35 gΒ·mol-1.
- Preparation of 2 mM stock solution (dissolved in H2O) for 2-mL O2k-chamber:
- Weigh 1.57 mg Ruthenium Red.
- Dissolve in 1 mL H2O.
- Divide into 0.1 mL aliquots.
- Store at -20 Β°C.
- Β» O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 1 Β΅L using a 10 Β΅L Hamilton syringe.
- Final concentration: 1 Β΅M.
- Preparation of 0.5 mM stock solution (dissolved in H2O) for 0.5-mL O2k-chamber:
- Take 50 Β΅L of the 1 mM stock solution.
- Add 150 Β΅L H2O.
- Β» O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (0.5-mL O2k-chamber): 1 Β΅L using a 10 Β΅L Hamilton syringe.
- Final concentration: 1 Β΅M.
References
- Amann R, Maggi CA (1991) Ruthenium red as a capsaicin antagonist.Life Sci. 9: 849-856.
- Israelson A, Zaid H, Abu-Hamad S, Nahon E, Shoshan-Barmatz V (2008) Mapping the ruthenium red-binding site of the voltage-dependent anion channel-1. Cell Calcium 43:196-204.
MitoPedia topics:
Inhibitor