Talk:ADP
From Bioblast
Preparation of 500 mM ADP stock solution (dissolved in H2O):
- 1) weigh 501.3 mg of ADP (FW = 501.3) = (1 mmol ADP)
- 2) add 1.2 ml H2O (ADP is not dissolved at this stage.
- 3) neutralize with 5 N KOH (approx. 450 Β΅l)! ADP will dissolve after addition of KOH!
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 2 ml and divide into 0.2 ml portions
- 6) store frozen at -80 Β°C
Preparation of 500 mM ADP stock solution with 3 mM free Mg2+ (dissolved in H2O):
To keep free [Mg2+] constant during respiration measurement mix ADP with MgCl2 (0.6 mol MgCl2/mol ADP).
MgCl2 (Scharlau MA0036: MgCl2.6H2O, FW = 203.3)
- 1) weigh 501.3 mg of ADP (FW = 501.3) = (1 mmol ADP)
- 2) add 1.2 ml H2O (ADP is not dissolved at this stage.
- 3) neutralize with 5 N KOH (approx. 450 Β΅l)! ADP will dissolve after addition of KOH!
- 4) add 121,98 mg MgCl2 .White precipitate will occur, which will be dissolved after 1-2 min of stirring on magnetic stirrer at room temperature!
- 5) check pH and adjust to 7 if necessary
- 6) adjust final volume to 2 ml and divide into 0.2 ml portions
- 7) store frozen at -80 Β°C
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