Additive effect of convergent electron flow: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''A''<sub>''α | |abbr=''A''<sub>''α&β''</sub> | ||
|description='''Additivity''' describes the princple of substrate control of mitochondrial respiration, where the '''additive effect of convergent CI | |description='''Additivity''' describes the princple of substrate control of mitochondrial respiration, where the '''additive effect of convergent CI&II electron flow''' is a consequence of electron flow converging at the '''Q-junction''' from respiratory Complexes I and II ([[CI&II e-input]]). Further additivity may be observed by convergent electron flow through [[glycerophosphate dehydrogenase]] and [[electron-transferring flavoprotein]]. Convergent electron flow corresponds to the operation of the [[TCA cycle]] and mitochondrial substrate supply ''in vivo''. Convergent electron flow simultaneously through Complexes I and II (CI&II) into the [[Q-junction]] supports higher [[OXPHOS capacity]] and [[ETS capacity]] than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI&II e-input are required for reconstitution of intracellular [[TCA cycle]] function. The convergent CI&II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with [[Mitochondrial preparations|mt-preparations]] have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to [[additivity]]. | ||
|info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger_2009_Int J Biochem Cell Biol]] | |info=[[Gnaiger 2014 MitoPathways]], [[Gnaiger_2009_Int J Biochem Cell Biol]] | ||
|type=Respiration | |type=Respiration | ||
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__TOC__ | __TOC__ | ||
= List of publications: CI and CII and CI | = List of publications: CI and CII and CI&II = | ||
{{#ask:[[Category:Publications]] [[Substrate states::CI]] [[Substrate states::CII]] [[Substrate states::CI+II]] | {{#ask:[[Category:Publications]] [[Substrate states::CI]] [[Substrate states::CII]] [[Substrate states::CI+II]] | ||
|?Was published in year=Year | |?Was published in year=Year | ||
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= List of publications: CI and CII and CI | = List of publications: CI and CII and CI&II and GpDH = | ||
{{#ask:[[Category:Publications]] [[Substrate states::CI]] [[Substrate states::CII]] [[Substrate states::CI+II]] [[Substrate states::GpDH]] | {{#ask:[[Category:Publications]] [[Substrate states::CI]] [[Substrate states::CII]] [[Substrate states::CI+II]] [[Substrate states::GpDH]] | ||
|?Was published in year=Year | |?Was published in year=Year | ||
Revision as of 14:04, 14 August 2014
- high-resolution terminology - matching measurements at high-resolution
Additive effect of convergent electron flow
Description
Additivity describes the princple of substrate control of mitochondrial respiration, where the additive effect of convergent CI&II electron flow is a consequence of electron flow converging at the Q-junction from respiratory Complexes I and II (CI&II e-input). Further additivity may be observed by convergent electron flow through glycerophosphate dehydrogenase and electron-transferring flavoprotein. Convergent electron flow corresponds to the operation of the TCA cycle and mitochondrial substrate supply in vivo. Convergent electron flow simultaneously through Complexes I and II (CI&II) into the Q-junction supports higher OXPHOS capacity and ETS capacity than separate electron flow through either CI or CII. Physiological substrate combinations supporting convergent CI&II e-input are required for reconstitution of intracellular TCA cycle function. The convergent CI&II effect may be completely or partially additive, suggesting that conventional bioenergetic protocols with mt-preparations have underestimated cellular OXPHOS capacities, due to the gating effect through a single branch, corresponding to additivity.
Abbreviation: Aα&β
Reference: Gnaiger 2014 MitoPathways, Gnaiger_2009_Int J Biochem Cell Biol
MitoPedia methods:
Respirometry
MitoPedia topics:
Substrate and metabolite
