Amplex UltraRed

high-resolution terminology - matching measurements at high-resolution

Amplex UltraRed

Description

Amplex red belongs to the extrinsic fluorophores and makes use of the fact that ROS generate hydrogen peroxide (H₂O₂) and it is the H₂O₂ level that Amplex red is used to measure. In the presence of horseradish peroxidase, Amplex red reacts with H₂O₂ to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H₂O₂ concentration.

Abbreviation: n.a.

Reference: Mishin 2010 Free Radical Biol. Med.

MitoPedia methods: Fluorometry