Difference between revisions of "Azide"
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'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: ≥50 µL using a | ::* Titration volume: ≥50 µL using a 100 µL syringe (2 mL O2k-Chamber). | ||
::* Final concentration: ≥100 mM. | ::* Final concentration: ≥100 mM. | ||
Revision as of 14:09, 27 August 2020
- high-resolution terminology - matching measurements at high-resolution
Azide
Description
Sodium azide is an inhbitor of cytochrome c oxidase (COX, CcO).
Abbreviation: Azd
Reference: MiPNet03.02
MitoPedia topics: Inhibitor
Application in HRR
Azd: Azide (Sodium azide, NaN3); Sigma S2002, SDS 25 g, store commercial stock at RT; FW = 65.01.
Caution: Very toxic!
Preparation of 4 M storage and stock solution (dissolved in H2O):
- 1) Weigh 260 mg of sodium azide.
- 2) Dissolve in 1 mL H20.
- 3) Divide into 0.5 mL portions.
- 4) Store at -20 °C.
O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: ≥50 µL using a 100 µL syringe (2 mL O2k-Chamber).
- Final concentration: ≥100 mM.
