Difference between revisions of "BAM15"
From Bioblast
(Created page with "'''2-fluorophenyl){6-[(2-fluorophenyl)amino](1,2,5-oxadiazolo[3,4-e]pyrazin-5-yl)}amine''' is a protonophore or uncoupler of oxidative phosphorylation detected in a screen fo...") ย |
|||
Line 1: | Line 1: | ||
'''2 | ย | ||
ย | |||
{{MitoPedia | |||
|abbr=DNP | |||
|description='''2,4-dinitrophenol''' (C<sub>6</sub>H<sub>4</sub>N<sub>2</sub>O<sub>5</sub>; FW = 184.11) is a protonophore acting as an [[uncoupler]] of [[Oxidative phosphorylation|oxidative phosphorylation]]. | |||
|info=[[Steinlechner-Maran 1996 Am J Physiol Cell Physiol]] | |||
|type=Uncoupler | |||
}} | |||
{{MitoPedia methods|type=Uncoupler | |||
}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Uncoupler | |||
|type=Uncoupler | |||
}} | |||
'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O): | |||
ย | |||
<span style="color:#8B008B"> '''Caution:''' Toxic!</span> | |||
ย | |||
::1. Weigh 3.7 mg of DNP (FW = 184.11). | |||
::2. Dissolve in 1.2 ml H2O. | |||
::3. Neutralize with 1 N KOH. | |||
::4. Adjust final volume to 2 ml and divide into 0.2 ml portions. | |||
::6. Store at -20 ยฐC. |
Revision as of 14:59, 21 May 2014
Description
2,4-dinitrophenol (C6H4N2O5; FW = 184.11) is a protonophore acting as an uncoupler of oxidative phosphorylation.
Abbreviation: DNP
Reference: Steinlechner-Maran 1996 Am J Physiol Cell Physiol
MitoPedia topics: Uncoupler
Preparation of 10 mM DNP stock solution (dissolved in H2O):
Caution: Toxic!
- 1. Weigh 3.7 mg of DNP (FW = 184.11).
- 2. Dissolve in 1.2 ml H2O.
- 3. Neutralize with 1 N KOH.
- 4. Adjust final volume to 2 ml and divide into 0.2 ml portions.
- 6. Store at -20 ยฐC.