Baeder 2016 Int J Dent: Difference between revisions
No edit summary |
Beno Marija (talk | contribs) No edit summary |
||
Line 15: | Line 15: | ||
|preparations=Permeabilized cells | |preparations=Permeabilized cells | ||
|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
| | |pathways=N, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2016-03, Amplex Red | |additional=2016-03, Amplex Red | ||
}} | }} |
Revision as of 15:30, 7 November 2016
ยป [[Has info::Open Access]]
Was written by::Baeder AC, Was written by::Napa K, Was written by::Richardson ST, Was written by::Taylor OJ, Was written by::Andersen SG, Was written by::Wilcox SH, Was written by::Winden DR, Was written by::Reynolds PR, Was written by::Bikman BT (Was published in year::2016) Was published in journal::Int J Dent
Abstract: [[has abstract::Cigarette smoke exposure compromises health through damaging multiple physiological systems, including disrupting metabolic function. The purpose of this study was to determine the role of oral gingiva in mediating the deleterious metabolic effects of cigarette smoke exposure on skeletal muscle metabolic function. Using an in vitro conditioned medium cell model, skeletal muscle cells were incubated with medium from gingival cells treated with normal medium or medium containing suspended cigarette smoke extract (CSE). Following incubation of muscle cells with gingival cell conditioned medium, muscle cell mitochondrial respiration and insulin signaling and action were determined as an indication of overall muscle metabolic health. Skeletal muscle cells incubated with conditioned medium of CSE-treated gingival cells had a profound reduction in mitochondrial respiration and respiratory control. Furthermore, skeletal muscle cells had a greatly reduced response in insulin-stimulated Akt phosphorylation and glycogen synthesis. Altogether, these results provide a novel perspective on the mechanism whereby cigarette smoke affects systemic metabolic function. In conclusion, we found that oral gingival cells treated with CSE create an altered milieu that is sufficient to both disrupted skeletal muscle cell mitochondrial function and insulin sensitivity.]] โข Keywords: has publicationkeywords::C2C12 mouse myoblast, has publicationkeywords::Myotubes
โข O2k-Network Lab: Was published by MiPNetLab::US UT Provo Bikman BT
Labels: MiParea: MiP area::Respiration, MiP area::Exercise physiology;nutrition;life style, MiP area::Pharmacology;toxicology
Organism: Organism::Mouse
Tissue;cell: tissue and cell::Skeletal muscle
Preparation: Preparation::Permeabilized cells
Coupling state: Coupling states::LEAK, Coupling states::OXPHOS, Coupling states::ETS
Pathway: Pathways::N, Pathways::NS, Pathways::ROX
HRR: Instrument and method::Oxygraph-2k