Difference between revisions of "Belosludtseva 2022 Membranes (Basel)"
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{{Publication | {{Publication | ||
|title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <sup>2+</sup>-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria. | |title=Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr <sup>2+</sup>-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria. https://doi.org/10.3390/membranes12070667 | ||
|info=Membranes (Basel) 12:667. [https://www.ncbi.nlm.nih.gov/pubmed/35877870 PMID: 35877870 Open Access] | |info=Membranes (Basel) 12:667. [https://www.ncbi.nlm.nih.gov/pubmed/35877870 PMID: 35877870 Open Access] | ||
|authors=Belosludtseva | |authors=Belosludtseva Natalia V, Pavlik Lyubov L, Belosludtsev Konstantin N, Saris Nils-Erik L, Shigaeva Maria I, Mironova Galina D | ||
|year=2022 | |year=2022 | ||
|journal=Membranes (Basel) | |journal=Membranes (Basel) | ||
|abstract=Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of | |abstract=Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr<sup>2+</sup> ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K<sup>+</sup> and Sr<sup>2+</sup> fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H<sub>2</sub>O<sub>2</sub> formation. The dynamic changes in the rate of H<sub>2</sub>O<sub>2</sub> production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca<sup>2+</sup>(Sr<sup>2+</sup>)-dependent phospholipase A<sub>2</sub> inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr<sup>2+</sup>-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr<sup>2+</sup>(Ca<sup>2+</sup>) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A<sub>2</sub> by the ions. A possible role for transient palmitate/Ca<sup>2+</sup>(Sr<sup>2+</sup>)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed. | ||
|keywords=Cyclosporin A, Cyclosporin A-independent palmitate/Ca2+-induced permeability transition pore, Ion oscillations, Lipid pore, Mitochondria, Mitochondrial permeability transition, Palmitic acid, Phospholipase A2 | |keywords=Cyclosporin A, Cyclosporin A-independent palmitate/Ca2+-induced permeability transition pore, Ion oscillations, Lipid pore, Mitochondria, Mitochondrial permeability transition, Palmitic acid, Phospholipase A2 | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=RU Pushchino Mironova GD | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|instruments=Oxygraph-2k | |organism=Rat | ||
|additional=2022-08 | |tissues=Liver | ||
|preparations=Isolated mitochondria | |||
|instruments=Oxygraph-2k, O2k-Fluorometer | |||
|additional=2022-08, AmR | |||
}} | }} |
Latest revision as of 16:35, 8 August 2022
Belosludtseva NV, Pavlik LL, Belosludtsev KN, Saris NL, Shigaeva MI, Mironova GD (2022) The short-term opening of cyclosporin A-independent palmitate/Sr 2+-induced pore can underlie ion efflux in the oscillatory mode of functioning of rat liver mitochondria. https://doi.org/10.3390/membranes12070667 |
Β» Membranes (Basel) 12:667. PMID: 35877870 Open Access
Belosludtseva Natalia V, Pavlik Lyubov L, Belosludtsev Konstantin N, Saris Nils-Erik L, Shigaeva Maria I, Mironova Galina D (2022) Membranes (Basel)
Abstract: Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A2 inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A2 by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed. β’ Keywords: Cyclosporin A, Cyclosporin A-independent palmitate/Ca2+-induced permeability transition pore, Ion oscillations, Lipid pore, Mitochondria, Mitochondrial permeability transition, Palmitic acid, Phospholipase A2 β’ Bioblast editor: Plangger M β’ O2k-Network Lab: RU Pushchino Mironova GD
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Liver
Preparation: Isolated mitochondria
HRR: Oxygraph-2k, O2k-Fluorometer
2022-08, AmR