Cardoso 2022 Abstract Bioblast: Difference between revisions
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{{Abstract | {{Abstract | ||
|title=Cardoso | |title=[[File:CardosoLHD.JPG|left|100px|Luiza Cardoso]] <u>Cardoso Luiza HD</u>, Doerrier C, Donnelly C, Komlรณdi T, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. Bioblast 2022: BEC Inaugural Conference. | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors=Cardoso Luiza HD, Doerrier Carolina, Donnelly Chris, Komlรณdi Timea, Gnaiger Erich | |authors=Cardoso Luiza HD, Doerrier Carolina, Donnelly Chris, Komlรณdi Timea, Gnaiger Erich | ||
|year=2022 | |year=2022 | ||
|event=Bioblast 2022 | |event=Bioblast 2022 | ||
|abstract= | |abstract=NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool โ defined as the sum of NAD(P)<sup>+</sup> and NAD(P)H โ and of the electron transfer system (ETS)-reactive coenzyme Q-pool [1] are linked in the N-pathway but are regulated independently by several convergent electron entries into the Q-junction [2]. Our results obtained with HRR and the NADH- and Q-Modules of the NextGen-O2k show that complementary to ET-pathway control of respiratory rate and redox state (redox push by electron input), coupling control exerts opposite effects on the metabolic parameters. Whereas redox push reduces the N- and Q-pool in the LEAK state at low O<sub>2</sub> flux and high protonomotive force ''pmF'', stimulation of O<sub>2</sub> flux by ADP in the OXPHOS state [3] is accompanied by a redox pull to the oxidized state. Under these conditions, ET-pathways converging at the Q-junction yield partial additivity of O<sub>2</sub> flux, when ET-capacity downstream of Q and phosphorylation capacity exert flux control [2]. Monitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitochondrial respiratory control at the N- and Q-junctions. | ||
<small> | <small> | ||
# Komlรณdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003 | # Komlรณdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003 | ||
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</small> | </small> | ||
|mipnetlab=AT Innsbruck Oroboros | |mipnetlab=AT Innsbruck Oroboros | ||
}} | }} | ||
== Affiliations and support == | == Affiliations and support == | ||
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:::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionโs Horizon 2020 research and innovation programme under grant agreement nยบ 859770. | :::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionโs Horizon 2020 research and innovation programme under grant agreement nยบ 859770. | ||
== Help == | == Help == |
Revision as of 04:25, 18 May 2022
Cardoso Luiza HD, Doerrier C, Donnelly C, Komlรณdi T, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. Bioblast 2022: BEC Inaugural Conference. |
Link: Bioblast 2022: BEC Inaugural Conference
Cardoso Luiza HD, Doerrier Carolina, Donnelly Chris, Komlรณdi Timea, Gnaiger Erich (2022)
Event: Bioblast 2022
NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool โ defined as the sum of NAD(P)+ and NAD(P)H โ and of the electron transfer system (ETS)-reactive coenzyme Q-pool [1] are linked in the N-pathway but are regulated independently by several convergent electron entries into the Q-junction [2]. Our results obtained with HRR and the NADH- and Q-Modules of the NextGen-O2k show that complementary to ET-pathway control of respiratory rate and redox state (redox push by electron input), coupling control exerts opposite effects on the metabolic parameters. Whereas redox push reduces the N- and Q-pool in the LEAK state at low O2 flux and high protonomotive force pmF, stimulation of O2 flux by ADP in the OXPHOS state [3] is accompanied by a redox pull to the oxidized state. Under these conditions, ET-pathways converging at the Q-junction yield partial additivity of O2 flux, when ET-capacity downstream of Q and phosphorylation capacity exert flux control [2]. Monitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitochondrial respiratory control at the N- and Q-junctions.
- Komlรณdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
- Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1
โข O2k-Network Lab: AT Innsbruck Oroboros
Affiliations and support
- Oroboros Instruments GmbH, Innsbruck, Austria
- This work was part of the Oroboros NextGen-O2k project, with funding from the European Unionโs Horizon 2020 research and innovation programme under grant agreement nยบ 859770.
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Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Liver, HEK
Preparation: Permeabilized cells, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k, NextGen-O2k