Difference between revisions of "Categories of SUIT protocols"

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high-resolution terminology - matching measurements at high-resolution

Categories of SUIT protocols

Description

Categories of SUIT protocols group SUIT protocols according to all substrate types involved in a protocol, independent of titrations of inhibitors which block the oxidation of the substrates present.

Abbreviation: SUITp-Catg

Template:MitoPedia SUIT

Categorization of SUIT protocols: substrate types

There are many ways to define groups of SUIT protocols. Since the complexity of SUIT protocols is primarily determined by the large number of possible substrate states, and to a lesser extent by the smaller number of possible coupling states, a relevant type of categories of SUIT protocols consideres the substrate types involved.

At the present stage of development of the 'library of SUIT protocols' as part of the MitoFit Quality Control System, five substrate types are considered:

On the pathway level of converging NADH- and FADH₂-linked dehydrogenases, including the TCA cycle and beta-oxidation:

N: NADH-linked substrates (CI-linked)

F: FADH₂-linked substrates (FAO)

On the pathway level of electron transfer complexes converging at the Q-junction:

S: Succinate (CII-linked)

Gp: Glycerophosphate (CGpDH-linked)

On the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer system:

Tm: Artificial electron transfer susbstrate TMPD (Tm) maintained in a reduced state by ascorbate (As) and reducing cytochrome c as the substrate of CIV.

SUITp Catg: Single substrate type

N
F
S
Gp
Tm

Tm can always included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.

SUITp-Categories: multiple substrate types

NS and more

NS

200px

NFS

200px

NFSGp

NFSGpTm

200px

N (without S) and more

NF

NGp

200px

1. FS
2. FSGp

@@ Line 9: / Line 9: @@
 }}
 {{MitoPedia topics}}
-== Substrate types ==
+__TOC__
+== Categorization of SUIT protocols: substrate types ==
+There are many ways to define groups of SUIT protocols. Since the complexity of SUIT protocols is primarily determined by the large number of possible [[substrate state]]s, and to a lesser extent by the smaller number of possible [[coupling state]]s, a relevant type of categories of SUIT protocols consideres the substrate types involved.
 At the present stage of development of the 'library of SUIT protocols' as part of the [[MitoFit Quality Control System]], five substrate types are considered:
@@ Line 22: / Line 25: @@
-== Categorization of SUIT protocols ==
+== SUITp Catg: Single substrate type ==
-There are many ways to define groups of SUIT protocols. Since the complexity of SUIT protocols is primarily determined by the large number of possible [[substrate state]]s, and to a lesser extent by the smaller number of possible [[coupling state]]s, a relevant type of categories of SUIT protocols consideres the substrate types involved.
-=== SUIT protocols with single substrate types ===
 # N
@@ Line 34: / Line 33: @@
 # Tm
-=== SUIT protocols with multiple substrate types ===
+Tm can always included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.
+== SUITp-Categories: multiple substrate types ==
-Tm can always included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.
+=== NS and more ===
+==== NS ====
+[[File:SUITp-Catg NS.jpg|200px]]
+==== NFS ====
+[[File:SUITp-Catg NFS.jpg|200px]]
+==== NFSGp ====
+[[File:SUITp-Catg NFSGp.jpg|200px]]
+==== NFSGpTm ====
+[[File:SUITp-Catg NFSGpTm.jpg|200px]]
+=== N (without S) and more ===
+==== NF ====
+[[File:SUITp-Catg NF.jpg|200px]]
+==== NGp ====
+[[File:SUITp-Catg NGp.jpg|200px]]
-==== Including N ====
-# NS
-# NFS
-# NFSGp
-==== Not including N ====
 ##FS
 ##FSGp