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Categories of SUIT protocols
Description
Categories of SUIT protocols group SUIT protocols according to all substrate types involved in a protocol, independent of titrations of inhibitors which block the oxidation of the substrates present.
Abbreviation: SUITp-Catg
Substrate types
At the present stage of development of the 'library of SUIT protocols' as part of the MitoFit Quality Control System, five substrate types are considered:
- On the pathway level of converging NADH- and FADH2-linked dehydrogenases, including the TCA cycle and beta-oxidation:
- N: NADH-linked substrates (CI-linked)
- F: FADH2-linked substrates (FAO)
- On the pathway level of electron transfer complexes converging at the Q-junction:
- S: Succinate (CII-linked)
- Gp: Glycerophosphate (CGpDH-linked)
- On the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer system:
- Tm: Artificial electron transfer susbstrate TMPD (Tm) maintained in a reduced state by ascorbate (Am) and reducing cytochrome c as the substrate of CIV.
