Categories of SUIT protocols

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Categories of SUIT protocols

Description

Categories of SUIT protocols group SUIT protocols according to all substrate types involved in a protocol, independent of titrations of inhibitors which block the oxidation of the substrates present.

Abbreviation: SUITp-Catg

Substrate types

At the present stage of development of the 'library of SUIT protocols' as part of the MitoFit Quality Control System, five substrate types are considered:

• On the pathway level of converging NADH- and FADH₂-linked dehydrogenases, including the TCA cycle and beta-oxidation:

N: NADH-linked substrates (CI-linked)

F: FADH₂-linked substrates (FAO)

• On the pathway level of electron transfer complexes converging at the Q-junction:

S: Succinate (CII-linked)

Gp: Glycerophosphate (CGpDH-linked)

• On the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer system:

Tm: Artificial electron transfer susbstrate TMPD (Tm) maintained in a reduced state by ascorbate (As) and reducing cytochrome c as the substrate of CIV.

Categorization of SUIT protocols

There are many ways to define groups of SUIT protocols. Since the complexity of SUIT protocols is primarily determined by the large number of possible substrate states, and to a lesser extent by the smaller number of possible coupling states, a relevant type of categories of SUIT protocols consideres the substrate types involved.

SUIT protocols with single substrate types

1. N
2. F
3. S
4. Gp
5. Tm

SUIT protocols with multiple substrate types

Tm can always included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.

Including N

1. NS
2. NFS
3. NFSGp

Not including N

1. 1. FS
   2. FSGp