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Categories of SUIT protocols

Description

Categories of SUIT protocols group SUIT protocols according to all ETS substrate types involved in a protocol, independent of titrations of inhibitors which block the oxidation of the substrates present. ROX states may or may not be included in a SUIT protocol, which does not change its category. » MiPNet article

Abbreviation: SUIT-Catg

Reference: MiPNet21.06 SUIT reference protocol

MitoPedia concepts: SUIT concept, "MitoFit Quality Control System" is not in the list (MiP concept, Respiratory state, Respiratory control ratio, SUIT concept, SUIT protocol, SUIT A, SUIT B, SUIT C, SUIT state, Recommended, ...) of allowed values for the "MitoPedia concept" property. MitoFit Quality Control System"MitoFit Quality Control System" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.

Categorization of SUIT protocols: ETS substrate types

Gnaiger E (2016) Categorization of SUIT protocols: ETS substrate types. MiPNet 2016-03-20.

» MiPNet21.06 SUIT reference protocol

OROBOROS (2016) MiPNet

Abstract: There are many ways to define groups of SUIT protocols. The complexity of SUIT protocols is primarily determined by the large number of possible substrate control states, compared to only three well defined coupling control states. Therefore, a relevant type of categories of SUIT protocols considers the ETS substrate types involved. Whereas the SUIT protocol names include all specific substrates applied in the SUIT protocol, the categories of SUIT protocols reduce this diversity to ETS substrate types

• O2k-Network Lab: AT Innsbruck OROBOROS

Labels: MiParea: Instruments;methods

HRR: Theory

Towards a library of SUIT protocols

At the present stage of development of the 'library of SUIT protocols' as part of the MitoFit Quality Control System, five ETS substrate types are considered.

N and F on the pathway level of converging NADH- and FADH₂-linked dehydrogenases, including the TCA cycle and beta-oxidation.
S and Gp on the pathway level of electron transfer complexes converging at the Q-junction.
Tm on the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer system. Tm can be included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.

SUIT-Catg: single substrate type

N - ETS-level 4: NADH-linked substrates (CI-linked)

F - ETS-level 4: FADH₂-linked substrates (FAO)

S - ETS-level 3: Succinate (CII-linked)

Gp - ETS-level 3: Glycerophosphate (CGpDH-linked)

Tm - ETS-level 1: Artificial electron transfer susbstrate TMPD (Tm) maintained in a reduced state by ascorbate (As) and reducing cytochrome c as the substrate of CIV.

SUIT-Catg: multiple ETS substrate types with NS

NS

NFS

NSGp

NFSGp

SUIT-Catg: multiple ETS substrate types with N (without S)

NF

NGp

NFGp

SUIT-Catg: multiple ETS substrate types without N

F and S without N are problematic substrate states due to accumulation of Oxa or Acetyl-CoA. Therefore, addition of malate alone (M without P or G) is not considered as substrate type N. However, high mt-malic enzyme activity requires a change of this concept, when M alone represents an ETS ccompetent substrate state. Low concentration of M may be used to support FAO, whereas a higher concentration of M may be required for N-linked respiratory capacity to override FAO capacity; this needs corresponding kinetic analyses (SUIT test protocols).

FS

FGp

SGp

FSGp