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Categories of SUIT protocols

Description

Categories of SUIT protocols group SUIT protocols according to all pathway control states involved in a protocol (controlling ETS pathway types; e.g. S, NS, FNS, FNSGp), independent of titrations of inhibitors which block the oxidation of specific substrate types. ROX states may or may not be included in a SUIT protocol, which does not change its category.

F - ETS-level 5: FADH₂-linked substrates (FAO) with obligatory support by the N-linked pathway.
N - ETS-level 4: NADH-linked substrates (CI-linked).
S - ETS-level 3: Succinate (CII-linked).
Gp - ETS-level 3: Glycerophosphate (CGpDH-linked).
CIV - ETS-level 1: Artificial electron transfer susbstrate TMPD (Tm) maintained in a reduced state by ascorbate (As) and reducing cytochrome c as the substrate of CIV.

» MiPNet article

Abbreviation: SUIT-catg

Reference: MiPNet21.06 SUIT RP

MitoPedia concepts: MiP concept, SUIT concept

Categorization of SUIT protocols: ETS pathway control states

Gnaiger E (2016) Categorization of SUIT protocols: MitoPathways. MiPNet 2016-03-20, edited 2016-08-21.

» MiPNet21.06 SUIT RP

OROBOROS (2016) MiPNet

Abstract: There are many ways to define groups of SUIT protocols. The complexity of SUIT protocols is primarily determined by the large number of possible pathway control states, compared to only three well defined coupling control states. Therefore, categories of SUIT protocols consider the ETS pathway control states involved. Whereas the SUIT protocol names include all specific substrates applied in the SUIT protocol, the categories of SUIT protocols reduce this diversity to pathway control states.

• O2k-Network Lab: AT Innsbruck OROBOROS

Labels: MiParea: Instruments;methods

Pathway: F, N, S, Gp, CIV, NS, Other combinations, ROX HRR: Theory

Towards a library of SUIT protocols

At the present stage of development of the Library of SUIT protocols as part of the MitoFit Quality Control System, five ETS pathway types are considered.

F-junction on the pathway level of converging FADH₂- and NADH-linked dehydrogenases, including beta-oxidationthe and segments of the TCA cycle.
N-junction on the pathway level of NADH-linked dehydrogenases, including the TCA cycle.
Q-junction on the pathway level of electron transfer complexes converging at the Q-junction (S and Gp).
Tm on the single step level of cytochrome c oxidase (CIV), the terminal step in the aerobic electron transfer system. Tm can be included or excluded at the end of a SUIT protocol. To simplify the categorization, Tm is not considered in this system of SUIT protocols.

SUIT-Catg: single pathway type

ETS-level 1: CIV

ETS-level 3: S, CII-pathway to Q

ETS-level 3: Gp, CGpDH-pathway to Q

ETS-level 4: N, N-junction or CI-pathway to Q

ETS-level 5: F, FAO, F-junction, CETF- supported by CI-pathway to Q

SUIT-catg: multiple ETS pathways with FNSGpCIV

SUIT-catg: multiple ETS pathways with NS

NS

FNS

NSGp

FNSGp

SUIT-catg: multiple ETS pathways with N (without S)

FN

NGp

FNGp

SUIT-catg: multiple ETS pathways without N

Addition of malate alone (M without P or G) is not considered as substrate type N. However, high mt-malic enzyme activity requires a change of this concept, when M alone represents an ETS ccompetent substrate state. Low concentration of M may be used to support FAO, whereas a higher concentration of M may be required for N-junction respiratory capacity to override FAO capacity; this needs corresponding kinetic analyses (SUIT test protocols).

FS

FGp

SGp

FSGp