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| '''General cleaning instructions:'''
| | #REDIRECT [[MiPNet19.03 O2k-cleaning and ISS]] |
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| There are at least three fundamentally different kind of contaminations that can accumulate in the measuring chamber of the oxygraph and cause problems. All of them have to be treated in different ways:
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| * [[Biological contamination]]: The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). If this does not help the biological contamination may be embedded in e.g. protein contamination and the glass chamber has to be disassembled and cleaned as described below:
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| * Protein contamination and other macroscopic deposits. After long use a whitish deposit can form at the glass walls of the chamber. Additionally, small glass splinters (difficult to see) may become embedded in such a deposit. This will be detected by a jumping stirring bar or a stirring bar getting stuck. In this case the glass chamber should be removed from the oxygraph and treated with concentrated hydrochloric acid at least over night. Use a stirring plate and the O2k stirring bar, in this way the stirring bar is cleaned simultaneously.
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| * [[Contamination by hydrophobic inhibitors]]: The ideal counter agent for this case is pure Ethanol (NOT 70% ethanol).
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| [[Talk:Cleaning the glass chamber|Further information >>]]
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| '''Cleaning between experiments:'''
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| For routine use of the O2k with the glass chambers inserted in the respirometer, cleaning should be done as explained in detail [[High-resolution_respirometry#Cleaning_the_chamber_between_experiments|here: Cleaning the chamber between experiments]]
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| {{#set: Technical service =Chamber}}
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| {{Technical service}}
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| __SHOWFACTBOX__
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