Difference between revisions of "Da Silva 2017 Life Sci"
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Copyright © 2017 Elsevier Inc. All rights reserved. | Copyright © 2017 Elsevier Inc. All rights reserved. | ||
|keywords=4-PBA, GLUT4, Glucose uptake, Hexokinase, L6 myotubes, Reactive oxygen species | |keywords=4-PBA, GLUT4, Glucose uptake, Hexokinase, L6 myotubes, Reactive oxygen species, Rat L6 myoblasts | ||
|editor=[[Kandolf G]], | |editor=[[Kandolf G]], | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, Pharmacology;toxicology | |area=Respiration, Pharmacology;toxicology | ||
|tissues=Skeletal muscle | |organism=Rat | ||
|tissues=Skeletal muscle, Other cell lines | |||
|preparations=Intact cells | |preparations=Intact cells | ||
|couplingstates=ROUTINE | |couplingstates=ROUTINE | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=2017-08, | ||
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Latest revision as of 12:45, 26 June 2019
da Silva MH, Peçanha FLM, de Oliveira AM, da-Silva WS (2017) 4-Phenyl butyric acid increases particulate hexokinase activity and protects against ROS injury in L6 myotubes. Life Sci 179:98-102. |
da Silva MH, Pecanha FLM, de Oliveira AM, da-Silva WS (2017) Life Sci
Abstract: Hexokinase (HK) is the first enzyme in the glycolytic pathway and is responsible for glucose phosphorylation and fixation into the cell. HK (HK-II) is expressed in skeletal muscle and can be found in the cytosol or bound mitochondria, where it can protect cells against insults such as oxidative stress. 4-Phenyl butyric acid (4-PBA) is a chemical chaperone that inhibits endoplasmic reticulum stress and contributes to the restoring of glucose homeostasis.
Here, we decided to investigate whether HK activity and its interaction with mitochondria could be a target of 4-PBA action.
L6 myotubes were treated with 1mM 4-PBA for 24, 48 or 72h. We evaluated HK activity, glucose and oxygen consumption, gene and protein expression.
We found that L6 myotubes treated with 4-PBA presented more HK activity in the particulate fraction, increased glucose consumption and augmented Glut4, Hk2 and Vdac1 mRNA expression. Moreover, 4-PBA prevented the deleterious effect of antimycin-A on HK particulate activity.
Together, these results suggest a new role of 4-PBA in glucose metabolism that includes HK as a potential target of beneficial effect of 4-PBA.
Copyright © 2017 Elsevier Inc. All rights reserved. • Keywords: 4-PBA, GLUT4, Glucose uptake, Hexokinase, L6 myotubes, Reactive oxygen species, Rat L6 myoblasts • Bioblast editor: Kandolf G
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Rat
Tissue;cell: Skeletal muscle, Other cell lines
Preparation: Intact cells
Coupling state: ROUTINE
HRR: Oxygraph-2k
2017-08