DatLab and SUIT protocols: Difference between revisions

Revision as of 16:51, 19 July 2016

high-resolution terminology - matching measurements at high-resolution

DatLab and SUIT protocols

Description

This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with SUIT protocols using the OROBOROS Oxygraph-2k and DatLab software. (1) Search for a specific SUIT protocol name (go to MitoPedia: SUIT). The list of MitoPedia SUIT protocols can be sorted by the SUIT protocol category (sorting by SUIT protocol name), which is listed as the 'abbreviation' of the SUIT protocol name. (2) Copy the template for Mark names into your DatLab subdirectory: DatLab\APPDATA\MTEMPLAT. (3) Copy the DatLab-Excel template for this SUIT protocol. (4) Follow the link to the corresponding publication or MiPNet communication, where the pdf file describing the SUIT protocol is available. After each sequential titration, a mark is set on the plot for flux or flow. After having set all marks, pull down the 'Mark names' menu, select the corresponding SUIT protocol for mark names, and rename all marks. The Mark names template also provides standard values of the titration volume preceding each mark. (5) Go to 'Mark statistics' [F2], copy to clipboard, and paste into the sample tab.

Reference: MitoPedia: DatLab

MitoPedia concepts: MiP concept, SUIT concept

Contributed by Gnaiger Erich 2016-07-19.

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 {{MitoPedia
 |abbr=
-|description=This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with '''[[SUIT protocols]]''' using the OROBOROS [[Oxygraph-2k]] and '''[[DatLab]]''' software. (1) Search for a specific [[SUIT protocol name]] (go to [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT]]) and follow the link to the corresponding publication or MiPNet communication.  The [[marks]] define the section of a [[respiratory state]] in the SUIT protocol.
+|description=This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with '''[[SUIT protocols]]''' using the OROBOROS [[Oxygraph-2k]] and '''[[DatLab]]''' software. (1) Search for a specific [[SUIT protocol name]] (go to [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT]]). The list of MitoPedia SUIT protocols can be sorted by the [[SUIT protocol category]] (sorting by SUIT protocol name), which is listed as the 'abbreviation' of the SUIT protocol name. (2) Copy the template for [[Mark names]] into your DatLab subdirectory: DatLab\APPDATA\MTEMPLAT. (3) Copy the [[DatLab-Excel templates |DatLab-Excel template]] for this SUIT protocol. (4) Follow the link to the corresponding publication or MiPNet communication, where the pdf file describing the SUIT protocol is available. After each sequential titration, a mark is set on the plot for flux or flow. After having set all marks, pull down the 'Mark names' menu, select the corresponding SUIT protocol for mark names, and rename all marks. The Mark names template also provides standard values of the titration volume preceding each mark. (5) Go to 'Mark statistics' [F2], copy to clipboard, and paste into the sample tab.
-The mark name contains the sequential number and the [[metabolic control variable]], ''X''. The metabolic control variable is the name of the preceding SUIT [[event]]. The [[MitoPedia: SUIT protocols |list of MitoPedia SUIT protocols]] can thus be sorted by the SUIT protocol category (sorting by SUIT protocol name), and by [[Subcategories of SUIT protocols |SUIT protocol subcategory]] which is listed as the abbreviation of the SUIT protocol name. The [[SUIT protocol pattern]] is best illustrated by a [[coupling-pathway control diagram]] (see Figure).
 |info=[[MitoPedia: DatLab]]
 }}

DatLab and SUIT protocols: Difference between revisions

Revision as of 16:51, 19 July 2016

Description

See also