De Meis 2010 PLoS One: Difference between revisions
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{{Publication | {{Publication | ||
|title= | |title=De Meis L, Ketzer LA, da Costa RM, de Andrade IR, Benchimol M (2010) Fusion of the endoplasmic reticulum and mitochondrial outer membrane in rats brown adipose tissue: activation of thermogenesis by Ca<sup>2+</sup>. PLoS One 5:e9439. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20209153 PMID: 20209153] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20209153 PMID: 20209153 Open Access] | ||
|authors= | |authors=De Meis L, Ketzer LA, da Costa RM, de Andrade IR, Benchimol M | ||
|year=2010 | |year=2010 | ||
|journal=PLoS | |journal=PLoS One | ||
|abstract=Brown adipose tissue (BAT) mitochondria thermogenesis is regulated by uncoupling protein 1 (UCP 1), GDP and fatty acids. In this report, we observed fusion of the endoplasmic reticulum (ER) membrane with the mitochondrial outer membrane of rats BAT. Ca<sup>2+</sup>-ATPase (SERCA 1) was identified by immunoelectron microscopy in both ER and mitochondria. This finding led us to test the Ca<sup>2+</sup> effect in BAT mitochondria thermogenesis. We found that Ca<sup>2+</sup> increased the rate of respiration and heat production measured with a microcalorimeter both in coupled and uncoupled mitochondria, but had no effect on the rate of ATP synthesis. The Ca<sup>2+</sup> concentration needed for half-maximal activation varied between 0.08 and 0.11 ยตM. The activation of respiration was less pronounced than that of heat production. Heat production and ATP synthesis were inhibited by rotenone and KCN. Liver mitochondria have no UCP1 and during respiration synthesize a large amount of ATP, produce little heat, GDP had no effect on mitochondria coupling, Ca<sup>2+</sup> strongly inhibited ATP synthesis and had little or no effect on the small amount of heat released. These | |abstract=Brown adipose tissue (BAT) mitochondria thermogenesis is regulated by uncoupling protein 1 (UCP 1), GDP and fatty acids. In this report, we observed fusion of the endoplasmic reticulum (ER) membrane with the mitochondrial outer membrane of rats BAT. Ca<sup>2+</sup>-ATPase (SERCA 1) was identified by immunoelectron microscopy in both ER and mitochondria. This finding led us to test the Ca<sup>2+</sup> effect in BAT mitochondria thermogenesis. We found that Ca<sup>2+</sup> increased the rate of respiration and heat production measured with a microcalorimeter both in coupled and uncoupled mitochondria, but had no effect on the rate of ATP synthesis. The Ca<sup>2+</sup> concentration needed for half-maximal activation varied between 0.08 and 0.11 ยตM. The activation of respiration was less pronounced than that of heat production. Heat production and ATP synthesis were inhibited by rotenone and KCN. Liver mitochondria have no UCP1 and during respiration synthesize a large amount of ATP, produce little heat, GDP had no effect on mitochondria coupling, Ca<sup>2+</sup> strongly inhibited ATP synthesis and had little or no effect on the small amount of heat released. These findings indicate that Ca<sup>2+</sup> activation of thermogenesis may be a specific feature of BAT mitochondria not found in other mitochondria such as liver. | ||
|keywords=Brown adipose tissue, Ca<sup>2+</sup>-ATPase, mt-membrane potential, Respiration, Heat Production, ATP Synthesis | |keywords=Brown adipose tissue, Ca<sup>2+</sup>-ATPase, mt-membrane potential, Respiration, Heat Production, ATP Synthesis | ||
|mipnetlab=BR Rio de Janeiro Galina A | |||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |||
|tissues=Fat | |||
|preparations=Isolated mitochondria | |||
|enzymes=Marker enzyme, Uncoupling protein | |||
|topics=Fatty acid | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} |
Latest revision as of 12:13, 26 May 2015
De Meis L, Ketzer LA, da Costa RM, de Andrade IR, Benchimol M (2010) Fusion of the endoplasmic reticulum and mitochondrial outer membrane in rats brown adipose tissue: activation of thermogenesis by Ca2+. PLoS One 5:e9439. |
De Meis L, Ketzer LA, da Costa RM, de Andrade IR, Benchimol M (2010) PLoS One
Abstract: Brown adipose tissue (BAT) mitochondria thermogenesis is regulated by uncoupling protein 1 (UCP 1), GDP and fatty acids. In this report, we observed fusion of the endoplasmic reticulum (ER) membrane with the mitochondrial outer membrane of rats BAT. Ca2+-ATPase (SERCA 1) was identified by immunoelectron microscopy in both ER and mitochondria. This finding led us to test the Ca2+ effect in BAT mitochondria thermogenesis. We found that Ca2+ increased the rate of respiration and heat production measured with a microcalorimeter both in coupled and uncoupled mitochondria, but had no effect on the rate of ATP synthesis. The Ca2+ concentration needed for half-maximal activation varied between 0.08 and 0.11 ยตM. The activation of respiration was less pronounced than that of heat production. Heat production and ATP synthesis were inhibited by rotenone and KCN. Liver mitochondria have no UCP1 and during respiration synthesize a large amount of ATP, produce little heat, GDP had no effect on mitochondria coupling, Ca2+ strongly inhibited ATP synthesis and had little or no effect on the small amount of heat released. These findings indicate that Ca2+ activation of thermogenesis may be a specific feature of BAT mitochondria not found in other mitochondria such as liver. โข Keywords: Brown adipose tissue, Ca2+-ATPase, mt-membrane potential, Respiration, Heat Production, ATP Synthesis
โข O2k-Network Lab: BR Rio de Janeiro Galina A
Labels:
Organism: Rat
Tissue;cell: Fat
Preparation: Isolated mitochondria
Enzyme: Marker enzyme, Uncoupling protein
Regulation: Fatty acid
Coupling state: OXPHOS
HRR: Oxygraph-2k