Diana 2015 Surg Innov

[[Has title::Diana M, Noll E, Diemunsch P, Moussallieh FM, Namer IJ, Charles AL, Lindner V, Agnus V, Geny B, Marescaux J (2015) Metabolism-guided bowel resection: potential role and accuracy of instant capillary lactates to identify the optimal resection site. Surg Innov 22:453-61.]]

» [[Has info::PMID: 26250483]]

Was written by::Diana M, Was written by::Noll E, Was written by::Diemunsch P, Was written by::Moussallieh FM, Was written by::Namer IJ, Was written by::Charles AL, Was written by::Lindner V, Was written by::Agnus V, Was written by::Geny B, Was written by::Marescaux J (Was published in year::2015) Was published in journal::Surg Innov

Abstract: [[has abstract::Strip-based handheld devices can measure lactatemia on capillary blood obtained by needle puncturing. We aimed to assess the kinetic of bowel capillary lactates, metabolomics profiling, and mitochondria respiratory rate in a prolonged model of bowel hypoperfusion.

In 6 pigs, a 3- to 4-cm ischemic segment was created in 6 small bowel loops (total = 36 loops) by clamping the vascular supply, for a duration of 1 to 6 hours. Hourly, 5 blood samples were obtained by puncturing the serosa, and lactates were measured using a handheld analyzer. Samples were made at the following regions of interest (ROIs): center of the ischemic area (1), proximal and distal clinical margins of resection (2a-2b), and vascularized zones (3a-3b). Every hour, surgical biopsies of ROIs were sampled. Activity of bowel mitochondria complexes was measured after 1, 3, and 5 hours of ischemia. Quantification of metabolites was performed on all samples (total N = 180).

Capillary lactates were significantly higher at ROI 1 versus ROI 3ab at all time points. After 1 hour lactates at the margins were significantly higher than those at vascularized areas (P = .0095), showing a mismatch between visual assessment and actual perfusion status. From 2 to 6 hours, there was no difference in lactates between ROIs 2a-2b and 3a-3b. Maximal tissue respiration decreased significantly after 1 hour (ROI 1 vs ROI 3ab). Seven metabolites (lactate, glucose, aspartate, choline, creatine, taurine, and tyrosine) expressed significantly different evolutions between ROIs.

Capillary lactates could help precisely estimate local bowel perfusion status.]] • Keywords: has publicationkeywords::Bowel hypoperfusion, has publicationkeywords::Capillary lactates, has publicationkeywords::Magnetic resonance spectroscopy, has publicationkeywords::Metabolomics profiling, has publicationkeywords::Oxygraphic mitochondria respiratory rate

• O2k-Network Lab: Was published by MiPNetLab::FR Strasbourg Zoll J

Labels: MiParea: MiP area::Respiration 

Stress:Injury and adaptation::Ischemia-reperfusion  Organism: Organism::Pig  Tissue;cell: tissue and cell::Endothelial;epithelial;mesothelial cell 

Coupling state: Coupling states::LEAK, Coupling states::OXPHOS  Pathway: Pathways::N, Pathways::S, Pathways::CIV  HRR: Instrument and method::Oxygraph-2k