Difference between revisions of "Drahota 2014 Physiol Res"
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{{Publication | {{Publication | ||
|title=Drahota Z, Palenickova E, Endlicher R, Milerova M, Brejchova J, Vosahlikova M, Svoboda P, Kazdova L, Kalous M, Cervinkova Z, Cahova M (2014) Biguanides inhibit complex I, II and IV of rat liver mitochondria and modify their functional properties. Physiol Res 63:1-11. | |title=Drahota Z, Palenickova E, Endlicher R, Milerova M, Brejchova J, Vosahlikova M, Svoboda P, Kazdova L, Kalous M, Cervinkova Z, Cahova M (2014) Biguanides inhibit complex I, II and IV of rat liver mitochondria and modify their functional properties. Physiol Res 63:1-11. | ||
| | |info=[http://www.ncbi.nlm.nih.gov/pubmed/24182344 PMID: 24182344] | ||
|authors=Drahota Z, Palenickova E, Endlicher R, Milerova M, Brejchova J, Vosahlikova M, Svoboda P, Kazdova L, Kalous M, Cervinkova Z, Cahova M | |authors=Drahota Z, Palenickova E, Endlicher R, Milerova M, Brejchova J, Vosahlikova M, Svoboda P, Kazdova L, Kalous M, Cervinkova Z, Cahova M | ||
|year=2014 | |year=2014 | ||
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and mitochondrial membrane potential. Direct measurements of | and mitochondrial membrane potential. Direct measurements of | ||
mitochondrial swelling revealed that phenformin increases the | mitochondrial swelling revealed that phenformin increases the | ||
resistance of the permeability transition pore to | resistance of the permeability transition pore to Ca<sup>2+<sup/> ions. Our | ||
data might be in agreement with the hypothesis of Schäfer | data might be in agreement with the hypothesis of Schäfer | ||
(1976) that binding of biguanides to membrane phospholipids | (1976) that binding of biguanides to membrane phospholipids | ||
| Line 29: | Line 29: | ||
considered as agents with high efficacy but low specifity. | considered as agents with high efficacy but low specifity. | ||
|keywords=Metformin, Phenformin, Mitochondrial enzymes, Mitochondrial permeability transition pore, Membrane fluidity | |keywords=Metformin, Phenformin, Mitochondrial enzymes, Mitochondrial permeability transition pore, Membrane fluidity | ||
|mipnetlab=CZ Hradec Kralove Cervinkova Z, CZ Prague Houstek J, CZ Prague Kalous M | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|organism=Rat | |||
|tissues=Liver | |||
|preparations=Homogenate, Isolated Mitochondria | |||
|couplingstates=OXPHOS | |||
|substratestates=CI, CII, CIV | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Revision as of 09:48, 17 December 2014
| Drahota Z, Palenickova E, Endlicher R, Milerova M, Brejchova J, Vosahlikova M, Svoboda P, Kazdova L, Kalous M, Cervinkova Z, Cahova M (2014) Biguanides inhibit complex I, II and IV of rat liver mitochondria and modify their functional properties. Physiol Res 63:1-11. |
Drahota Z, Palenickova E, Endlicher R, Milerova M, Brejchova J, Vosahlikova M, Svoboda P, Kazdova L, Kalous M, Cervinkova Z, Cahova M (2014) Physiol Res
Abstract: In this study, we focused on an analysis of biguanides effects on mitochondrial enzyme activities, mitochondrial membrane potential and membrane permeability transition pore function. We used phenformin, which is more efficient than metformin, and evaluated its effect on rat liver mitochondria and isolated hepatocytes. In contrast to previously published data, we found that phenformin, after a 5 min pre-incubation, dose-dependently inhibits not only mitochondrial complex I but also complex II and IV activity in isolated mitochondria. The enzymes complexes inhibition is paralleled by the decreased respiratory control index and mitochondrial membrane potential. Direct measurements of mitochondrial swelling revealed that phenformin increases the resistance of the permeability transition pore to Ca2+ ions. Our data might be in agreement with the hypothesis of Schäfer (1976) that binding of biguanides to membrane phospholipids alters membrane properties in a non-specific manner and, subsequently, different enzyme activities are modified via lipid phase. However, our measurements of anisotropy of fluorescence of hydrophobic membrane probe diphenylhexatriene have not shown a measurable effect of membrane fluidity with the 1 mM concentration of phenformin that strongly inhibited complex I activity. Our data therefore suggest that biguanides could be considered as agents with high efficacy but low specifity. • Keywords: Metformin, Phenformin, Mitochondrial enzymes, Mitochondrial permeability transition pore, Membrane fluidity
• O2k-Network Lab: CZ Hradec Kralove Cervinkova Z, CZ Prague Houstek J, CZ Prague Kalous M
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Liver
Preparation: Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Coupling state: OXPHOS
HRR: Oxygraph-2k
