FCCP: Difference between revisions

Revision as of 14:29, 22 November 2010

high-resolution terminology - matching measurements at high-resolution

FCCP

Description

Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C₁₀H₅F₃N₄O); uncoupler, F(x): FCCP added at concentration x.

Abbreviation: Fx

Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP

Labels:

Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.

HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.

Application in HRR

F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C₁₀H₅F₃N₄O); Sigma C 2920, 10 mg, store at 4 °C: FW = 254.17)

Caution: Very toxic. Chemicals stored in the fridge or freezer should be adjusted to room temperature before opening.

Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):

1) weigh 1.27 mg of FCCP directly into a 5 ml glass vial

2) add 5 ml Ethanol

3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution

4) store in glass vials (recommendation) at -20 °C

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl steps using a 10 µl syringe
Final conc. in 2 ml O2k-chamber: 0.5 µM steps

@@ Line 11: / Line 11: @@
 |type=Uncoupler
 }}
 == Application in [[HRR]] ==
@@ Line 19: / Line 18: @@
-'''Preparation of 4 mg/ml stock solution''' (dissolved in H<sub>2</sub>0):
+'''Preparation of 1 mM stock solution''' (dissolved in 99.9% Ethanol):
-::1) weigh 4 mg of Malonic acid directly into a 2 ml glass vial
+::1) weigh 1.27 mg of FCCP directly into a 5 ml glass vial
-::2) add 1 ml H<sub>2</sub>0
+::2) add 5 ml Ethanol
-::3) divide into 0.2 ml portions
+::3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
-::4) store frozen at -20 °C
+::4) store in glass vials (recommendation) at -20 °C
 '''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]