Difference between revisions of "Fluorescence-Sensor"
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Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See [[MiPNet12.06]] for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers. | Use only [[Stopper\black PEEK\conical Shaft\central Port|black PEEK stoppers]] in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See [[MiPNet12.06]] for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers. | ||
'''O2k-Manual: [[ | '''O2k-Manual: [[O2k-Fluorescence_LED2-Module#Electronic_settings|Electronic settings]]''' |
Revision as of 01:47, 12 March 2012
Fluorescence-Sensor
Description | Fluorescence-Sensor: LED with specified wavelength, photodiode, Filter-Cap attached with specific optical filter for the LED and/or photodiode. See Fluorescence-Sensor Green and Fluorescence-Sensor Cyan. |
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Product ID | 442## |
Type | O2k, MultiSensor, O2k-Fluorescence LED2-Module, O2k-Manual"O2k-Manual" is not in the list (O2k, O2k-Module, O2k-Core, O2k-Fluorometer, O2k-Main Unit, O2k-Assembly Kit, OroboPOS, OroboPOS-Service Kit, ISS, TIP2k, ...) of allowed values for the "Product type" property. |
Link | O2k-Fluorescence LED2-Module |
Image |
O2k-Manual
Select the Fluorescence-Sensors
Switching between different excitation wavelengths and filters is achieved by simply exchanging the fluorescence sensors. Two types of optical sensors are supplied with different LEDs for fluorescence excitation, and the effective spectra of the LEDs are modified by filters:
- LED 525 nm wavelength: Green. The installed Filter-Cap is ready for H2O2 measurement with Amplex® UltraRed.
- LED 480 nm wavelength: Cyan. The installed Filter-Cap is ready for measurements with Magnesium green® or Calcium green®. A different filter is used for measurement of mt-membrane potential with safranin.
Setup - next step: Mounting a Filter-Cap
Connect Fluorescence-Sensor to O2k
For recording of fluorescence signals, insert the black sensor into the window of the O2k chamber as far as possible, aligning the Sensor-Guide Sector with the Sensor-Guide of the O2k-Front Fixation. Then connect the cable of the Fluorescence-Sensor to the Fluorescence-Sensor Plug of the Fluorescence-Control Unit.
Stoppers
Use only black PEEK stoppers in conjunction with fluorometric measurements. If necessary, replace the previous white PVDF stoppers. The black stoppers can be used for all HRR applications in general. See MiPNet12.06 for calibration of the O2k-chamber volume, which is identical for PEEK and PVDF stoppers.
O2k-Manual: Electronic settings