Difference between revisions of "Friederich-Persson 2013 Abstract MiP2013"
Beno Marija (talk | contribs) |
|||
| (7 intermediate revisions by 4 users not shown) | |||
| Line 1: | Line 1: | ||
{{Abstract | {{Abstract | ||
|title=Friederich-Persson M, Hansell P, Wilcox CS, Palm F (2013) Potassium controls rat mitochondrial function: ''In vivo'' and ''in vitro'' considerations. Mitochondr Physiol Network 18.08. | |title=Friederich-Persson M, Hansell P, Wilcox CS, Palm F (2013) Potassium controls rat mitochondrial function: ''In vivo'' and ''in vitro'' considerations. Mitochondr Physiol Network 18.08. | ||
|info=[[File:Logo MiP2013.jpg|150px|right|MiPsociety]][[MiP2013]], [[Laner 2013 Mitochondr Physiol Network MiP2013|Book of Abstracts Open Access]] | |||
|authors=Friederich-Persson M, Hansell P, Wilcox CS, Palm F | |authors=Friederich-Persson M, Hansell P, Wilcox CS, Palm F | ||
|year=2013 | |year=2013 | ||
|event= | |event=MiPNet18.08_MiP2013 | ||
|abstract=The intracellular potassium concentration, [K+], varies depending on tissue type and is about 60 mM in kidney proximal tubular cells whereas it is approximately 120 mM in cardiac myocytes. Intracellular [K+] may therefore affect mitochondria function. | |abstract=The intracellular potassium concentration, [K+], varies depending on tissue type and is about 60 mM in kidney proximal tubular cells whereas it is approximately 120 mM in cardiac myocytes. Intracellular [K+] may therefore affect mitochondria function. | ||
| Line 15: | Line 16: | ||
|organism=Rat | |organism=Rat | ||
|tissues=Heart, Kidney | |tissues=Heart, Kidney | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|topics=Flux control, Ion;substrate transport | |topics=Flux control, Ion;substrate transport | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=MiP2013 | |additional=MiP2013, S06 | ||
}} | }} | ||
== Affiliations and author contributions == | == Affiliations and author contributions == | ||
1 - Dept of Medical Cell Biology, Uppsala University, Sweden | 1 - Dept of Medical Cell Biology, Uppsala University, Sweden | ||
Latest revision as of 11:11, 28 April 2017
| Friederich-Persson M, Hansell P, Wilcox CS, Palm F (2013) Potassium controls rat mitochondrial function: In vivo and in vitro considerations. Mitochondr Physiol Network 18.08. |
Link:
MiP2013, Book of Abstracts Open Access
Friederich-Persson M, Hansell P, Wilcox CS, Palm F (2013)
Event: MiPNet18.08_MiP2013
The intracellular potassium concentration, [K+], varies depending on tissue type and is about 60 mM in kidney proximal tubular cells whereas it is approximately 120 mM in cardiac myocytes. Intracellular [K+] may therefore affect mitochondria function.
By measuring the respiratory function and swelling of mitochondria isolated from kidney cortex and heart at [K+] ranging from 15 mM to 146 mM we showed that [K+] controls mitochondria function in the heart. Increasing [K+] resulted in increased resting state respiration and decreased respiratory control ratio (RCR) in kidney cortex mitochondria. The reduced RCR corresponded with mitochondria swelling, indicating failing mitochondria. The effects of K+ in kidney mitochondria was prevented by inhibitors of ATP-sensitive K+-channels and voltage-gated K+-channels (glibenclamide and by 4-aminopyridine, respectively). On the contrary, RCR in heart mitochondria increased with increasing [K+] and was unaffected by K+channel inhibitors.
These results demonstrate [K+] control of mitochondria function in rat kidney and heart.
• O2k-Network Lab: SE Uppsala Liss P
Labels: MiParea: Respiration, mt-Structure;fission;fusion
Organism: Rat
Tissue;cell: Heart, Kidney
Preparation: Isolated mitochondria
Regulation: Flux control, Ion;substrate transport
HRR: Oxygraph-2k
MiP2013, S06
Affiliations and author contributions
1 - Dept of Medical Cell Biology, Uppsala University, Sweden
2 - Dept of Medicine, Division of Hypertension and Nephrology, Georgetown University Medical Center, Washington DC, USA;
3 - Dept of Medical and Health Sciences, Linköping University, Sweden;
4 - Center for Medical Image Science and Visualization, Linköping University, Sweden.
Email: [email protected]
