Glucose: Difference between revisions

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|abbr=Glc
|abbr=Glc
|description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
|description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
|info=[http://www.oroboros.at/index.php?id=yeast_hrr-reference-assay MiPNet15.09]
|info=[[MiPNet15.09. Yeast: HRR Reference Assay]]
}}
}}
{{MitoPedia concepts}}
{{MitoPedia methods}}
{{MitoPedia O2k and high-resolution respirometry}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Substrate and metabolite
|mitopedia topic=Substrate and metabolite
}}
}}
== Application in [[HRR]]ย  ==
== Application in [[HRR]]ย  ==



Revision as of 09:30, 2 June 2016


high-resolution terminology - matching measurements at high-resolution


Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay






MitoPedia topics: Substrate and metabolite 

Application in HRR

Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at R.T.; FW = 180.16


Preparation of 2 M stock solution (dissolved in H2O):

1) weigh 360.32 mg of Glucose
2) dissolve in 1 ml H2O


Oxygraph-2k Titration:

  • Titration volume: 20 ยตl using a 50 ยตl syringe
  • Final conc. in 2 ml O2k-chamber: 20 mM
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