Glucose: Difference between revisions
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|abbr=Glc | |abbr=Glc | ||
|description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate. | |description='''Glucose''', also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate. | ||
|info=[ | |info=[[MiPNet15.09. Yeast: HRR Reference Assay]] | ||
}} | }} | ||
== Application in [[HRR]]ย = | __TOC__ | ||
== Application in [[HRR]] == | |||
{{Chemical_description | |||
|abbr=Glc | |||
|trivial name=Glucose | |||
|complete name=D-(+)-Glucose | |||
|chem formula=C<sub>6</sub>H<sub>12</sub>0<sub>6</sub> | |||
|molar mass=180.16 | |||
|vendor=Sigma-Aldrich | |||
|product number=G8270 | |||
|store at=RT | |||
|sensitivity= | |||
|cas=50-99-7 | |||
|h statements= | |||
|h info= | |||
}}<!--:::'''Glc:ย Glucose''' (D-(+)-Glucose,ย C<sub>6</sub>H<sub>12</sub>0<sub>6</sub>); Sigma G 8270, 100 g, store at RT; M = 180.16 gยทmol<sup>-1</sup>--> | |||
:::: 2021-03: The preparation instructions were updated to take the volume of the solute into account (see: [[Volume_of_the_solute| Volume of the solute]]). | |||
ย | |||
ย | |||
::::'''Preparation of 2 M stock solution''' (1 mL, dissolved in H<sub>2</sub>O): | |||
ย | |||
::::# weigh 360.32 mg of Glucose | |||
::::# dissolve in 775 ยตL H<sub>2</sub>O in 1.5 mL Eppendorf | |||
ย | |||
:::ยป'''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
ย | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 20 ยตL using a 50 ยตL Hamilton syringe. | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 5 ยตL using a 25 ยตL Hamilton syringe. | |||
::::* Final concentration: 20 mM | |||
ย | |||
ย | |||
::::'''Preparation of 2.2 M stock solution''' (200 ยตL, dissolved in H<sub>2</sub>O): | |||
::::# weigh 79.2 mg of Glucose ย | |||
::::# dissolve 155 ยตL H<sub>2</sub>O in 0.5 mL Eppendorf | |||
:::ยป'''O2k manual titrations'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 ยตL using a 25 ยตL Hamilton syringe. | |||
::::* Final concentration: 5.5 mM (1 g/L) | |||
''' | ::::* Titration volume ('''2-mL O2k-chamber'''): 10 ยตL using a 25 ยตL Hamilton syringe. | ||
::::* Final concentration: 11 mM (2 g/L) | |||
<!-- | |||
::::* Titration volume: 5 ยตL using a 25 ยตL syringe | |||
::::* Final conc. in 2 mL O2k-chamber: 5.5 mM (1 g/L) | |||
:: | ::::* Titration volume: 10 ยตL using a 25 ยตL syringe | ||
::2 | ::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ||
--> | |||
{{MitoPedia concepts}} | |||
{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics | |||
|mitopedia topic=Substrate and metabolite | |||
}} |
Latest revision as of 12:11, 15 June 2021
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose; C6H1206), Sigma-Aldrich: G8270, store at RT, CAS: 50-99-7, M = 180.16 gยทmol-1
- 2021-03: The preparation instructions were updated to take the volume of the solute into account (see: Volume of the solute).
- Preparation of 2 M stock solution (1 mL, dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve in 775 ยตL H2O in 1.5 mL Eppendorf
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 20 ยตL using a 50 ยตL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 5 ยตL using a 25 ยตL Hamilton syringe.
- Final concentration: 20 mM
- Preparation of 2.2 M stock solution (200 ยตL, dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 155 ยตL H2O in 0.5 mL Eppendorf
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 ยตL using a 25 ยตL Hamilton syringe.
- Final concentration: 5.5 mM (1 g/L)
- ยปO2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 10 ยตL using a 25 ยตL Hamilton syringe.
- Final concentration: 11 mM (2 g/L)
MitoPedia topics:
Substrate and metabolite