Gnaiger 1998 BTK-HRR: Difference between revisions
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{{Publication | {{Publication | ||
|title=Gnaiger E, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R (1998) High-resolution respirometry. Optimum permeabilization of the cell membrane by digitonin. In BioThermoKinetics in the Post Genomic Era (Larsson C, | |title=Gnaiger E, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R (1998) High-resolution respirometry. Optimum permeabilization of the cell membrane by digitonin. In BioThermoKinetics in the Post Genomic Era (Larsson C, Pahlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:89-95. | ||
|authors=Gnaiger | |info=[[Media:BTK_89-95_HRR.pdf| '''BTK 1998: 89-95 pdf'']] | ||
|authors=Gnaiger Erich, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R | |||
|year=1998 | |year=1998 | ||
| | |journal=Chalmers Reproservice | ||
|abstract=Permeabilization of the cell membrane is becoming an established alternative to the isolation of mitochondria in bioenergetic studies of cultured cells and biopsy samples of muscle. Depending on cell type, 5 to 50 million cells are required for a respirometric measurement of endogenous and permeabilized-cell respiration [1,2], using conventional oxygen monitoring instruments. An order of magnitude less cells are required when using | |abstract=Permeabilization of the cell membrane is becoming an established alternative to the isolation of mitochondria in bioenergetic studies of cultured cells and biopsy samples of muscle. Depending on cell type, 5 to 50 million cells are required for a respirometric measurement of endogenous and permeabilized-cell respiration [1,2], using conventional oxygen monitoring instruments. An order of magnitude less cells are required when using high-resolution respirometry [3] (Tab. 1), which provides an up-to-date standard for general and clinical bioenergetics, including the diagnosis of mitochondrial defects, testing of drugs, oxidative stress and hypoxia-reoxygenation injury [4-6]. We provide an overview of (i) the specific features of high-resolution respirometry, (ii) test experiments for instrumental evaluation, and (iii) digitonin titrations for determining optimum concentrations for cell membrane permeabilization in cultured cells. | ||
test experiments for instrumental evaluation, and (iii) digitonin titrations for determining optimum concentrations for cell membrane permeabilization in cultured cells. | |mipnetlab=AT Innsbruck Gnaiger E, DE Regensburg Renner-Sattler K | ||
| | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k, TIP2k | |area=Respiration, Instruments;methods | ||
|organism=Human | |||
|tissues=Endothelial;epithelial;mesothelial cell, HUVEC | |||
|preparations=Intact cells, Permeabilized cells | |||
|topics=Inhibitor, Oxygen kinetics, Substrate | |||
|couplingstates=ROUTINE, OXPHOS | |||
|pathways=S | |||
|instruments=Oxygraph-2k, TIP2k | |||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
}} | }} | ||
Latest revision as of 04:26, 23 November 2021
| Gnaiger E, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R (1998) High-resolution respirometry. Optimum permeabilization of the cell membrane by digitonin. In BioThermoKinetics in the Post Genomic Era (Larsson C, Pahlman I-L, Gustafsson L, eds) Chalmers Reproservice, Göteborg:89-95. |
Gnaiger Erich, Kuznetsov AV, Lassnig B, Fuchs A, Reck M, Renner K, Stadlmann S, Rieger G, Margreiter R (1998) Chalmers Reproservice
Abstract: Permeabilization of the cell membrane is becoming an established alternative to the isolation of mitochondria in bioenergetic studies of cultured cells and biopsy samples of muscle. Depending on cell type, 5 to 50 million cells are required for a respirometric measurement of endogenous and permeabilized-cell respiration [1,2], using conventional oxygen monitoring instruments. An order of magnitude less cells are required when using high-resolution respirometry [3] (Tab. 1), which provides an up-to-date standard for general and clinical bioenergetics, including the diagnosis of mitochondrial defects, testing of drugs, oxidative stress and hypoxia-reoxygenation injury [4-6]. We provide an overview of (i) the specific features of high-resolution respirometry, (ii) test experiments for instrumental evaluation, and (iii) digitonin titrations for determining optimum concentrations for cell membrane permeabilization in cultured cells.
• O2k-Network Lab: AT Innsbruck Gnaiger E, DE Regensburg Renner-Sattler K
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Tissue;cell: Endothelial;epithelial;mesothelial cell, HUVEC
Preparation: Intact cells, Permeabilized cells
Regulation: Inhibitor, Oxygen kinetics, Substrate Coupling state: ROUTINE, OXPHOS Pathway: S HRR: Oxygraph-2k, TIP2k
