Gnaiger 2008 POS

Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [pdf]

Contents

1. Introduction
2. Polarographic oxygen sensor and traditional oxygraphy
3. High-resolution respirometry: the Oxygraph-2k
   1. Calibration of polarographic oxygen sensors and oxygen concentration in respiration media at air saturation
   2. From oxygraph slopes to respiratory flux corrected for background effects
4. Phosphorylation control protocol with intact cells
   1. Titration steps of the PC protocol
   2. Experimental example for the PC protocol
   3. Flux control ratios from the PC protocol
5. Intact cells, permeabilized cells and tissue, or isolated mitochondria
   1. Intact cells
   2. Permeabilized cells and tissue
   3. Isolated mitochondria
6. Titration protocols in permeabilized cells, permeabilized tissue preparations, and isolated mitochondria
7. Multisensor applications

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