Difference between revisions of "Gnaiger 2012 Abstract Bioblast-Gentle Science"
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The strategy of OROBOROS INSTRUMENTS included the close link between university and company, teaching and training with Open Access to our published [3] and unpublished scientific background information, detailed descriptions of [http://www.oroboros.at/?O2k-protocols O2k-Protocols] on our website, and direct communication with our customers and O2k-users ([[MiPNet_Reference_Laboratories|Mitochondrial Physiology Network]]). | The strategy of OROBOROS INSTRUMENTS included the close link between university and company, teaching and training with Open Access to our published [3] and unpublished scientific background information, detailed descriptions of [http://www.oroboros.at/?O2k-protocols O2k-Protocols] on our website, and direct communication with our customers and O2k-users ([[MiPNet_Reference_Laboratories|Mitochondrial Physiology Network]]). | ||
Conforming to the [http://www.oroboros.at/?Oroboros-logo OROBOROS logo] we are proud to receive highly positive [[O2k-Feedback]], but also provide help with [[O2k-Troubleshooting]] in open discussions. Since a [http://www.oroboros.at/?Mitopathways-group 2007 workshop in Schroecken] and the first [http://www.mitophysiology.org/?MiP-summerschool2007 MiPsummer school 2007], advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the '[[Gnaiger 2007 MitoPathways|Blue Book]]'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) ''protocols developed by us'' without proper citation. | Conforming to the [http://www.oroboros.at/?Oroboros-logo OROBOROS logo] we are proud to receive highly positive [[O2k-Feedback]], but also provide help with [[O2k-Troubleshooting]] in open discussions. Since a [http://www.oroboros.at/?Mitopathways-group 2007 workshop in Schroecken] and the first [http://www.mitophysiology.org/?MiP-summerschool2007 MiPsummer school 2007], advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the '[[Gnaiger 2007 MitoPathways|Blue Book]]'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) "''protocols developed by us''" without proper citation. | ||
Gentle Science is an illusion worthwhile to maintain. | |||
# [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]] | # [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]] | ||
# [[Gnaiger 2001 Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]] | # [[Gnaiger 2001 Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]] | ||
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== Further references == | == Further references == | ||
* [[Gnaiger 1994 BTK|Gnaiger E, Gellerich FN, Wyss M (1994) What is Controlling Life? 50 years after Erwin Schrödinger's What is Life? Modern Trends in BiothermoKinetics 3, Innsbruck Univ Press, 336 pp.]] | * [[Gnaiger 1994 BTK|Gnaiger E, Gellerich FN, Wyss M (1994) What is Controlling Life? 50 years after Erwin Schrödinger's What is Life? Modern Trends in BiothermoKinetics 3, Innsbruck Univ Press, 336 pp.]] | ||
* [[Haller T, Ortner M, Gnaiger E (1994) A respirometer for investigating oxidative cell metabolism. Toward optimization of respiratory studies. Analyt Biochem 218: 338-342.]] - An instrumental description of the first generation ''Oxygraph'' was provided by the 'Cyclobios' research group collaborating in the [http://www.oroboros.at/?Oroboros1989 FWF Project P7162-BIO]. | |||
* [[Gnaiger 2007 MitoPathways|Gnaiger E, ed (2007) Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck, 1st ed: 96 pp.]] | * [[Gnaiger 2007 MitoPathways|Gnaiger E, ed (2007) Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck, 1st ed: 96 pp.]] | ||
* The term Gentle Science was coined by [[http://www.bioblast.at/index.php/Talk:Gentle_Science#Shilpa_Iyer_2010-01-01|Shilpa Iyer (2009)]]. | |||
== Help == | == Help == | ||
* [[Abstracts help]] | * [[Abstracts help]] | ||
* [[MitoPedia Glossary: Terms and abbreviations]] | * [[MitoPedia Glossary: Terms and abbreviations]] |
Revision as of 06:51, 23 October 2012
Gnaiger E (2012) Gentle Science and the Bioblast Conference. Mitochondr Physiol Network 17.12. |
Link: MiPNet17.12 Bioblast Conference - Open Access
Gnaiger E (2012)
Event: Bioblast Conference on Mitochondrial Competence
The concept of Gentle Science has been a driving force for more than 20 years of continuous development of high-resolution respirometry, which was initiated in a FWF project (1989). Our first generation Oxygraph was commercially available in 1992. A series of applications was presented at a BTK conference in 1994, and a full description of the instrument, method and concept of high-resolution respirometry was published in 1995 [1], when the positive feedback from early users had confirmed the validity of our approach [2]. The concept of O2k-MultiSensor extensions was implemented in the second generation Oxygraph-2k, developed in close cooperation with Phlipp Gradl (WGT-Elektronik) and distributed since 2002.
The strategy of OROBOROS INSTRUMENTS included the close link between university and company, teaching and training with Open Access to our published [3] and unpublished scientific background information, detailed descriptions of O2k-Protocols on our website, and direct communication with our customers and O2k-users (Mitochondrial Physiology Network).
Conforming to the OROBOROS logo we are proud to receive highly positive O2k-Feedback, but also provide help with O2k-Troubleshooting in open discussions. Since a 2007 workshop in Schroecken and the first MiPsummer school 2007, advanced SUIT protocols for sequential activation particularly of CI-, CI+II- and CII-linked respiration are explained in the 'Blue Book'. It is not in the spirit of Gentle Science, however, to receive enthusiastic feedback by Emails and then read in a publication (2008) "protocols developed by us" without proper citation.
Gentle Science is an illusion worthwhile to maintain.
- Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.
- Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.
• Keywords: High-resolution respirometry, Gentle Science, Oxygraph-2k
• O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck Gnaiger E
Labels:
HRR: Oxygraph-2k, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Affiliations and author contributions
OROBOROS INSTRUMENTS, Innsbruck, Austria; and D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck, Austria. Email: [email protected]
Further references
- Gnaiger E, Gellerich FN, Wyss M (1994) What is Controlling Life? 50 years after Erwin Schrödinger's What is Life? Modern Trends in BiothermoKinetics 3, Innsbruck Univ Press, 336 pp.
- Haller T, Ortner M, Gnaiger E (1994) A respirometer for investigating oxidative cell metabolism. Toward optimization of respiratory studies. Analyt Biochem 218: 338-342. - An instrumental description of the first generation Oxygraph was provided by the 'Cyclobios' research group collaborating in the FWF Project P7162-BIO.
- Gnaiger E, ed (2007) Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications, Innsbruck, 1st ed: 96 pp.
- The term Gentle Science was coined by [Iyer (2009)].