Gnaiger 2020 BEC MitoPathways: Difference between revisions
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|authors=Gnaiger E | |authors=Gnaiger E | ||
|year=2012 | |year=2012 | ||
|abstract= | |abstract= | ||
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|keywords=ETS, Q-junction, respiratory states, flux control ratios | |keywords=ETS, Q-junction, respiratory states, flux control ratios | ||
|mipnetlab=AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS | |mipnetlab=AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS | ||
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|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
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__TOC__ | |||
= Supplementary information = | |||
* '''Preface''' | |||
== Chapter 1. OXPHOS analysis == | |||
== Chapter 2. Mitochondrial pathways to Complex I: Respiratory substrate control with pyruvate, malate and glutamate == | |||
=== Notes - Pitfalls === | |||
2.1. Schwerzmann et al (1989) Proc Natl Acad Sci U S A 86: 1583-1587. βOf the substrates used here, pyruvate/malate activates the chain at complex I, glutamate/malate and succinate at complexes II and III, ..β | |||
2.2. Ponsot et al (2005) J Cell Physiol 203: 479-486. (a) Respiration (State 3) in permeabilized fibres with malate alone gave 25-50% of the flux with pyruvate+malate. This most likely indicates a large content of endogenous mitochondrial substrates, which interfere to an unknown degree with the kinetics of respiration after addition of exogenous substrates. In such a study, the conventional initial depletion of endogenous substrates would be most important. (b) Maximal respiration rates in muscle should be evaluated at saturating or high Pi, since at a Pi concentration of 3 mM OXPHOS respiration is phosphate limited. | |||
2.3. Hulbert et al (2006) J Comp Physiol B 176: 93-105. Addition of βsparking malate concentrationsβ. This term can probably be derived from the misconception that tricarboxylic acid cycle intermediates are conserved during respiration of isolated mitochondria. 380 Β΅M malate (instead of mM concentrations) in conjunction with 2.4 mM pyruvate were used, which makes a comparison difficult between different tissues and different species: the low malate concentration may limit PMP flux at various degrees in the different sources of mitochondria, and GMP may support higher fluxes than PMP at tissue- and species-specific degrees. | |||
=== References Chapter 2 === | |||
== Chapter 3. Mitochondrial pathways to Complex II. Glycerophosphate dehydrogenase and electrontransferring flavoprotein == | |||
== Chapter 4. Mitochondrial pathways to Complexes I+II:Β Convergent electron transfer at the Q-Junction and additive effect of substrate combinations | |||
== Chapter 5. Respiratory states, coupling control and coupling control ratios | |||
== Chapter 6. Conversions of metabolic fluxes | |||
== Apendix == | |||
=== A1. Respiratory coupling states and coupling control ratios === | |||
=== A2. Substrates, uncouplers and inhibitors === |
Revision as of 09:14, 25 November 2012
Gnaiger E ed (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. Mitochondr Physiol Network 17.##: ## pp. 3rd ed. ISBN 978-3-9502399-6-6 |
Β» MiPNet2007
Gnaiger E (2012)
Abstract: β’ Keywords: ETS, Q-junction, respiratory states, flux control ratios
β’ O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS
Labels:
Organism: Human, Mouse
Tissue;cell: Skeletal muscle, Fibroblast
Preparation: Permeabilized cells, Permeabilized tissue, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Mitochondrial Biogenesis; Mitochondrial Density"Mitochondrial Biogenesis; Mitochondrial Density" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Theory, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Supplementary information
- Preface
Chapter 1. OXPHOS analysis
Chapter 2. Mitochondrial pathways to Complex I: Respiratory substrate control with pyruvate, malate and glutamate
Notes - Pitfalls
2.1. Schwerzmann et al (1989) Proc Natl Acad Sci U S A 86: 1583-1587. βOf the substrates used here, pyruvate/malate activates the chain at complex I, glutamate/malate and succinate at complexes II and III, ..β 2.2. Ponsot et al (2005) J Cell Physiol 203: 479-486. (a) Respiration (State 3) in permeabilized fibres with malate alone gave 25-50% of the flux with pyruvate+malate. This most likely indicates a large content of endogenous mitochondrial substrates, which interfere to an unknown degree with the kinetics of respiration after addition of exogenous substrates. In such a study, the conventional initial depletion of endogenous substrates would be most important. (b) Maximal respiration rates in muscle should be evaluated at saturating or high Pi, since at a Pi concentration of 3 mM OXPHOS respiration is phosphate limited. 2.3. Hulbert et al (2006) J Comp Physiol B 176: 93-105. Addition of βsparking malate concentrationsβ. This term can probably be derived from the misconception that tricarboxylic acid cycle intermediates are conserved during respiration of isolated mitochondria. 380 Β΅M malate (instead of mM concentrations) in conjunction with 2.4 mM pyruvate were used, which makes a comparison difficult between different tissues and different species: the low malate concentration may limit PMP flux at various degrees in the different sources of mitochondria, and GMP may support higher fluxes than PMP at tissue- and species-specific degrees.
References Chapter 2
Chapter 3. Mitochondrial pathways to Complex II. Glycerophosphate dehydrogenase and electrontransferring flavoprotein
== Chapter 4. Mitochondrial pathways to Complexes I+II: Convergent electron transfer at the Q-Junction and additive effect of substrate combinations
== Chapter 5. Respiratory states, coupling control and coupling control ratios
== Chapter 6. Conversions of metabolic fluxes