Hecker 2015 Methods Mol Biol: Difference between revisions
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monocytes were pretreated with TNF-ฮฑ to mimic infl ammation followed by incubation with SCFA/MCFA | monocytes were pretreated with TNF-ฮฑ to mimic infl ammation followed by incubation with SCFA/MCFA | ||
and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR. | and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR. | ||
|keywords=Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-ฮฑ | |keywords=Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-ฮฑ, HUVEC | ||
|mipnetlab=DE Giessen Weissmann N | |mipnetlab=DE Giessen Weissmann N | ||
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|topics=Coupling efficiency;uncoupling, Uncoupler, Fatty acid | |topics=Coupling efficiency;uncoupling, Uncoupler, Fatty acid | ||
|couplingstates=LEAK, ROUTINE, ETS | |couplingstates=LEAK, ROUTINE, ETS | ||
|substratestates= | |substratestates=ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} | ||
Revision as of 11:20, 14 July 2016
| Hecker M, Sommer N, Mayer K (2015) Assessment of short- and medium-chain fatty acids on mitochondrial function in severe Inflammation. Methods Mol Biol 1265:389-96. |
Hecker M, Sommer N, Mayer K (2015) Methods Mol Biol
Abstract: Mitochondrial dysfunction is regarded as one key factor involved in the pathogenesis of septic disorders, leading to a decline in energy supply. The infl uence of short- and medium-chain fatty acids (SCFA/MCFA) on mitochondrial respiration under infl ammatory conditions has thus far not been investigated. In the following protocol, we describe the assessment of mitochondrial respiration using high-resolution respirometry under infl ammatory and baseline conditions. For this approach, human endothelial cells and monocytes were pretreated with TNF-ฮฑ to mimic infl ammation followed by incubation with SCFA/MCFA and then subjected to high-resolution respirometry. Mitochondrial DNA content was assessed by PCR. โข Keywords: Mitochondria, Respiration, Oxygen consumption, SCFA, MCFA, TNF-ฮฑ, HUVEC
โข O2k-Network Lab: DE Giessen Weissmann N
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, mtDNA;mt-genetics
Pathology: Sepsis
Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Intact cells
Regulation: Coupling efficiency;uncoupling, Uncoupler, Fatty acid Coupling state: LEAK, ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
