Difference between revisions of "Hoeks 2011 PLoS One"
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|title=Hoeks J, de Wilde J, Hulshof MF, van den Berg SA, Schaart G, van Dijk KW, Smit E, Mariman EC (2011) High fat diet-induced changes in mouse muscle mitochondrial phospholipids do not impair mitochondrial respiration despite insulin resistance. PLoS One 6: e27274. ย | |title=Hoeks J, de Wilde J, Hulshof MF, van den Berg SA, Schaart G, van Dijk KW, Smit E, Mariman EC (2011) High fat diet-induced changes in mouse muscle mitochondrial phospholipids do not impair mitochondrial respiration despite insulin resistance. PLoS One 6: e27274. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/22140436 PMID: 22140436] ย | |info=[http://www.ncbi.nlm.nih.gov/pubmed/22140436 PMID: 22140436] | ||
|authors=Hoeks J, de Wilde J, Hulshof MF, van den Berg SA, Schaart G, van Dijk KW, Smit E, Mariman EC | |authors=Hoeks J, de Wilde J, Hulshof MF, van den Berg SA, Schaart G, van Dijk KW, Smit E, Mariman EC | ||
|year=2011 | |year=2011 | ||
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|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|organism=Mouse | |organism=Mouse | ||
|tissues=Skeletal | |tissues=Skeletal muscle | ||
|preparations=Isolated Mitochondria | |preparations=Isolated Mitochondria | ||
|topics=Respiration; OXPHOS; ETS Capacity | |topics=Respiration; OXPHOS; ETS Capacity | ||
}} | }} |
Revision as of 03:30, 5 April 2012
Hoeks J, de Wilde J, Hulshof MF, van den Berg SA, Schaart G, van Dijk KW, Smit E, Mariman EC (2011) High fat diet-induced changes in mouse muscle mitochondrial phospholipids do not impair mitochondrial respiration despite insulin resistance. PLoS One 6: e27274. |
Hoeks J, de Wilde J, Hulshof MF, van den Berg SA, Schaart G, van Dijk KW, Smit E, Mariman EC (2011) PLoS One
Abstract: BACKGROUND: Type 2 diabetes mellitus and muscle insulin resistance have been associated with reduced capacity of skeletal muscle mitochondria, possibly as a result of increased intake of dietary fat. Here, we examined the hypothesis that a prolonged high-fat diet consumption (HFD) increases the saturation of muscle mitochondrial membrane phospholipids causing impaired mitochondrial oxidative capacity and possibly insulin resistance.
METHODOLOGY: C57BL/6J mice were fed an 8-week or 20-week low fat diet (10 kcal%; LFD) or HFD (45 kcal%). Skeletal muscle mitochondria were isolated and fatty acid (FA) composition of skeletal muscle mitochondrial phospholipids was analyzed by thin-layer chromatography followed by GC. High-resolution respirometry was used to assess oxidation of pyruvate and fatty acids by mitochondria. Insulin sensitivity was estimated by HOMA-IR.
PRINCIPAL FINDINGS: At 8 weeks, mono-unsaturated FA (16โถ1n7, 18โถ1n7 and 18โถ1n9) were decreased (-4.0%, p<0.001), whereas saturated FA (16โถ0) were increased (+3.2%, p<0.001) in phospholipids of HFD vs. LFD mitochondria. Interestingly, 20 weeks of HFD descreased mono-unsaturated FA while n-6 poly-unsaturated FA (18โถ2n6, 20โถ4n6, 22โถ5n6) showed a pronounced increase (+4.0%, p<0.001). Despite increased saturation of muscle mitochondrial phospholipids after the 8-week HFD, mitochondrial oxidation of both pyruvate and fatty acids were similar between LFD and HFD mice. After 20 weeks of HFD, the increase in n-6 poly-unsaturated FA was accompanied by enhanced maximal capacity of the electron transport chain (+49%, p = 0.002) and a tendency for increased ADP-stimulated respiration, but only when fuelled by a lipid-derived substrate. Insulin sensitivity in HFD mice was reduced at both 8 and 20 weeks.
CONCLUSIONS/INTERPRETATION: Our findings do not support the concept that prolonged HF feeding leads to increased saturation of skeletal muscle mitochondrial phospholipids resulting in a decrease in mitochondrial fat oxidative capacity and (muscle) insulin resistance. โข Keywords: skeletal muscle, mouse, mitochondrial phospholipids, high resolution respirometry
โข O2k-Network Lab: NL_Maastricht_Schrauwen P
Labels:
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k