Komlodi 2021 MitoFit Q: Difference between revisions
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{{Publication | {{Publication | ||
|title=Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2 [[doi:10.26124/mitofit:2021-0002]]. | |title=Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2 [[doi:10.26124/mitofit:2021-0002]]. | ||
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|year=2021-02-18 | |year=2021-02-18 | ||
|journal=MitoFit Prep | |journal=MitoFit Prep | ||
|abstract= Redox states of mitochondrial coenzyme Q (mtCoQ or Q) reflect the balance between (''1'') reducing capacities of electron flow from fuel substrates converging at the Q-junction, (''2'') oxidative capacities downstream of Q to oxygen, and (''3'') the load on the OXPHOS system utilizing or dissipating the protonmotive force. A three-electrode sensor (Rich 1988; Moore et al 1988) was implemented into the NextGen-O2k to monitor the Q redox state continuously and simultaneously with oxygen consumption. The Q-Module was optimized for high signal-to-noise ratio and minimum oxygen diffusion. CoQ2 is added as a redox probe equilibrating with Q at Complexes CI, CII and CIII and the detecting electrode. Q-sensors are poised with the CoQ2 redox peak potentials determined by cyclic voltammetry, which provides quality control of the Q-sensor and reveals chemical interferences. | |abstract=Redox states of mitochondrial coenzyme Q (mtCoQ or Q) reflect the balance between (''1'') reducing capacities of electron flow from fuel substrates converging at the Q-junction, (''2'') oxidative capacities downstream of Q to oxygen, and (''3'') the load on the OXPHOS system utilizing or dissipating the protonmotive force. A three-electrode sensor (Rich 1988; Moore et al 1988) was implemented into the NextGen-O2k to monitor the Q redox state continuously and simultaneously with oxygen consumption. The Q-Module was optimized for high signal-to-noise ratio and minimum oxygen diffusion. CoQ2 is added as a redox probe equilibrating with Q at Complexes CI, CII and CIII and the detecting electrode. Q-sensors are poised with the CoQ2 redox peak potentials determined by cyclic voltammetry, which provides quality control of the Q-sensor and reveals chemical interferences. | ||
The Q redox state and oxygen consumption were measured simultaneously in isolated mitochondria. A coupling-control protocol was applied to analyze LEAK, OXPHOS, and electron transfer capacities (''L'', ''P'', and ''E'', respectively) in the succinate-pathway. In a second pathway-control protocol, NADH- and succinate-linked pathways (N and S) converge at the Q-junction. mtCoQ was more oxidized when O<sub>2</sub> flux was stimulated in coupling-control states with load increasing from ''L'' to ''P'' and ''E''. In contrast, mtCoQ was more reduced when O<sub>2</sub> flux was stimulated with electron input capacities increasing from N-, S- to NS-pathway-control states. N- and S- pathway capacities were not completely additive, thus confirming partial pool behavior of Q as proposed in the plasticity model of supercomplex organization. | The Q redox state and oxygen consumption were measured simultaneously in isolated mitochondria. A coupling-control protocol was applied to analyze LEAK, OXPHOS, and electron transfer capacities (''L'', ''P'', and ''E'', respectively) in the succinate-pathway. In a second pathway-control protocol, NADH- and succinate-linked pathways (N and S) converge at the Q-junction. mtCoQ was more oxidized when O<sub>2</sub> flux was stimulated in coupling-control states with load increasing from ''L'' to ''P'' and ''E''. In contrast, mtCoQ was more reduced when O<sub>2</sub> flux was stimulated with electron input capacities increasing from N-, S- to NS-pathway-control states. N- and S- pathway capacities were not completely additive, thus confirming partial pool behavior of Q as proposed in the plasticity model of supercomplex organization. | ||
|keywords=Q-junction, mitochondria, oxygen consumption, Q redox state, three-electrode system, cyclic voltammetry, harmonized SUIT protocols, high-resolution respirometry, coupling control, pathway control, NS-pathway, additivity | |keywords=Q-junction, mitochondria, oxygen consumption, Q redox state, three-electrode system, cyclic voltammetry, harmonized SUIT protocols, high-resolution respirometry, coupling control, pathway control, NS-pathway, additivity | ||
|editor= | |editor=Komlodi T | ||
|mipnetlab=AT Innsbruck Oroboros | |mipnetlab=AT Innsbruck Oroboros | ||
}} | }} | ||
{{Labeling | |||
|additional=SUIT-006 Q mt D071, SUIT-006 Q ce-pce D073, SUIT-031 Q mt D072, SUIT-031 Q ce-pce D074, MitoFit 2021 Photosynthesis | |||
}} | |||
{{MitoFit page name}} | |||
ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9876-1411]] Komlodi Timea, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-6392-9229]] Cardoso Luiza HD, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich | ORC'''ID''': [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-9876-1411]] Komlodi Timea, [[File:ORCID.png|20px|link=https://orcid.org/0000-0001-6392-9229]] Cardoso Luiza HD, [[File:ORCID.png|20px|link=https://orcid.org/0000-0002-7969-6762]] Doerrier Carolina, [[File:ORCID.png|20px|link=https://orcid.org/0000-0003-3647-5895]] Gnaiger Erich | ||
__TOC__ | __TOC__ | ||
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== Cited by == | == Cited by == | ||
{{Template:Cited by Huete-Ortega M 2021 MitoFit Photosynthesis protocols}} | {{Template:Cited by Huete-Ortega M 2021 MitoFit Photosynthesis protocols}} | ||
Revision as of 00:00, 19 February 2021
| [[Has title::Komlodi T, Cardoso LHD, Doerrier C, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. MitoFit Preprints 2021.2 doi:10.26124/mitofit:2021-0002.]] |
» [[Has info::
Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria]]
Was written by::Komlodi Timea, Was written by::Cardoso Luiza HD, Was written by::Doerrier Carolina, Was written by::Gnaiger Erich (Was published in year::2021-02-18) Was published in journal::MitoFit Prep
Abstract: [[has abstract::Redox states of mitochondrial coenzyme Q (mtCoQ or Q) reflect the balance between (1) reducing capacities of electron flow from fuel substrates converging at the Q-junction, (2) oxidative capacities downstream of Q to oxygen, and (3) the load on the OXPHOS system utilizing or dissipating the protonmotive force. A three-electrode sensor (Rich 1988; Moore et al 1988) was implemented into the NextGen-O2k to monitor the Q redox state continuously and simultaneously with oxygen consumption. The Q-Module was optimized for high signal-to-noise ratio and minimum oxygen diffusion. CoQ2 is added as a redox probe equilibrating with Q at Complexes CI, CII and CIII and the detecting electrode. Q-sensors are poised with the CoQ2 redox peak potentials determined by cyclic voltammetry, which provides quality control of the Q-sensor and reveals chemical interferences. The Q redox state and oxygen consumption were measured simultaneously in isolated mitochondria. A coupling-control protocol was applied to analyze LEAK, OXPHOS, and electron transfer capacities (L, P, and E, respectively) in the succinate-pathway. In a second pathway-control protocol, NADH- and succinate-linked pathways (N and S) converge at the Q-junction. mtCoQ was more oxidized when O2 flux was stimulated in coupling-control states with load increasing from L to P and E. In contrast, mtCoQ was more reduced when O2 flux was stimulated with electron input capacities increasing from N-, S- to NS-pathway-control states. N- and S- pathway capacities were not completely additive, thus confirming partial pool behavior of Q as proposed in the plasticity model of supercomplex organization.]] • Keywords: has publicationkeywords::Q-junction, has publicationkeywords::mitochondria, has publicationkeywords::oxygen consumption, has publicationkeywords::Q redox state, has publicationkeywords::three-electrode system, has publicationkeywords::cyclic voltammetry, has publicationkeywords::harmonized SUIT protocols, has publicationkeywords::high-resolution respirometry, has publicationkeywords::coupling control, has publicationkeywords::pathway control, has publicationkeywords::NS-pathway, has publicationkeywords::additivity • Bioblast editor: has editor::Komlodi T • O2k-Network Lab: Was published by MiPNetLab::AT Innsbruck Oroboros
Labels:
additional label::SUIT-006 Q mt D071, additional label::SUIT-006 Q ce-pce D073, additional label::SUIT-031 Q mt D072, additional label::SUIT-031 Q ce-pce D074, additional label::MitoFit 2021 Photosynthesis
Komlodi 2021 MitoFit Q
ORCID: 
Komlodi Timea, Cardoso Luiza HD, Doerrier Carolina, Gnaiger Erich
References
{{#ask:Additional label::MitoFit 2021 CoQ | mainlabel=Link |?Has title=Reference |?Has info=View |?Was published in year=Year |format=broadtable |limit=5000 |offset=0 |sort=Has title |order=ascending }}
Keywords
- Bioblast links: Q - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coenzyme Q
- » Coenzyme Q
- » Quinone, Ubiquinone Q; oxidized
- » Quinol, Ubiquinol QH2; reduced
- » Semiquinone
- » Coenzyme Q2
- » Q-redox state
- » Q-pools
- Coenzyme Q
- Mitochondrial pathways, respiratory Complexes, and Q
- » Q-cycle
- » Q-junction
- » Convergent electron flow
- » NS-pathway
- » FNS
- » FNSGp
- Mitochondrial pathways, respiratory Complexes, and Q
- NextGen-O2k and Q-Module
Cited by
- Huete-Ortega et al (2021) Substrate-uncoupler-inhibitor-titration protocols for photosynthesis in Chlamydomonas reinhardtii. MitoFit Preprints 2021 (in prep).
