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Difference between revisions of "MiPNet06.05 Test Experiments on O2k-Specifications"

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{{Publication
{{Publication
|title=Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr. Physiol. Network 6.5.
|title=Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5.
|info=[http://www.oroboros.at/index.php?id=o2k-specifications MiPNet06.05]
|info=[http://www.oroboros.at/index.php?id=o2k-specifications MiPNet06.05]
|authors=Gnaiger E,  
|authors=Gnaiger E,
|year=*
|year=*
|journal=Mitochondr. Physiol. Network
|journal=Mitochondr Physiol Network
|abstract=Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term “high-resolution respirometry”: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 μM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 °C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system.
|abstract=Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term “high-resolution respirometry”: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 μM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 °C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system.
|mipnetlab=AT Innsbruck OROBOROS,
|mipnetlab=AT Innsbruck OROBOROS,

Revision as of 10:36, 22 June 2012

Publications in the MiPMap
Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5.

» MiPNet06.05

Gnaiger E (

) Mitochondr Physiol Network

Abstract: Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term “high-resolution respirometry”: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 μM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 °C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system.


O2k-Network Lab: AT Innsbruck OROBOROS


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HRR: Oxygraph-2k, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.