Difference between revisions of "MiPNet06.05 Test Experiments on O2k-Specifications"
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|title=Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5. | |title=Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5. | ||
|info=[http://www.oroboros.at/ | |info=[http://www.oroboros.at/?o2k-specifications MiPNet06.05] | ||
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|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
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|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
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Revision as of 11:33, 10 August 2013
| Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5. |
Abstract: Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term “high-resolution respirometry”: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 μM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 °C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system.
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
HRR: Oxygraph-2k
