MiPNet06.05 Test Experiments on O2k-Specifications

Gnaiger E (2001) Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr Physiol Network 6.5.

» Open Access-MiPNet06.05.pdf; www O2k-Specifications

OROBOROS (2001) Mitochondr Physiol Network

Abstract: Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term “high-resolution respirometry”: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 μM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 °C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system.

• O2k-Network Lab: AT Innsbruck OROBOROS

Labels: MiParea: Instruments;methods 

HRR: Oxygraph-2k 

• Version 1: 2001
• Version 10: 2010-08-20