Difference between revisions of "MiPNet06.05 Test Experiments on O2k-Specifications"
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|title=Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr. Physiol. Network 6.5. | |||
|info=[http://www.oroboros.at/index.php?id=o2k-specifications&no_cache=1&sword_list[]=06.05 MiPNet06.05] | |||
|authors=Gnaige rE | |||
|year=* | |||
|journal=Mitochondr. Physiol. Network | |||
|abstract=Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term “high-resolution respirometry”: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 μM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 °C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system. | |||
|mipnetlab=AT Innsbruck OROBOROS, | |||
}} | |||
{{Labeling | |||
|instruments=Oxygraph-2k, MiPNet-Publication | |||
}} |
Revision as of 15:26, 14 September 2011
Gnaiger E. Test experiments on specifications of the OROBOROS Oxygraph. Mitochondr. Physiol. Network 6.5. |
» [=06.05 MiPNet06.05]
) Mitochondr. Physiol. Network
Abstract: Summary: The OROBOROS Oxygraph with DatLab software is world-wide the only instrument on the market which allows routine measurements to be made with specifications summarized under the term “high-resolution respirometry”: The limit of detection of O2 flux is as low as 0.5 pmols-1cm-3. Signal noise at zero oxygen concentration is <0.05 μM O2. Oxygen backdiffusion at zero oxygen is <3 pmols-1cm-3, and oxygen consumption at air saturation and standard barometric pressure (100 kPa) was 2.7 +/- 0.9 SD in 114 test runs at 37 °C. These highly standardized instrumental background fluxes are a linear function of oxygen concentration, which is used for routine background correction of oxygen flux. Typical exponential time constants of the oxygen sensors are <4 s, used for dynamic corrections in kinetic studies. Results of an extensive experimental test program are presented as the basis of OROBOROS Oxygraph specifications, which distinguish high-resolution respirometry from any conventional oxygraph system.
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
HRR: Oxygraph-2k, MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.