Difference between revisions of "MiPNet09.12 O2k-Titrations"
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== Note: Titration of chemicals in respirometry == | |||
Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water. | |||
== Further information == | == Further information == | ||
::::» [[MitoPedia: SUIT]] | ::::» [[MitoPedia: SUIT]] - Substrate-uncoupler-inhibitor-titration (SUIT) protocols for respirometry | ||
::::» [[MiPNet03.02 Chemicals-Media|MiPNet03.02]] - Chemicals and media for respirometry | |||
::::» [[MiPNet19.14 SOP Hamilton microsyringes |MiPNet19.14]] - SOP for manual O2k-titrations with Hamilton microsyringes. | ::::» [[MiPNet19.14 SOP Hamilton microsyringes |MiPNet19.14]] - SOP for manual O2k-titrations with Hamilton microsyringes. | ||
Revision as of 11:19, 27 April 2017
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OROBOROS (2016-09-30) Mitochondr Physiol Network
Abstract: OROBOROS (2016) Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(15): 1-2. »Versions
A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock concentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.
- » O2k-Protocols
- » Product: OROBOROS O2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Regulation: ADP, Inhibitor, Substrate, Uncoupler, Amino acid
HRR: O2k-Protocol
O2k-chemicals and media
Note: Titration of chemicals in respirometry
Overshoots or short interruptions of the flux after the injection of chemicals do not have a meaning in respirometric measurements. A disturbance of the signal is only observed due to the injected chemical and does not represent mitochondrial respiration. With the addition of a chemical solved in ethanol a little bit of oxygen is always introduced, since ethanol has higher oxygen solubility than water.
Further information
- » MitoPedia: SUIT - Substrate-uncoupler-inhibitor-titration (SUIT) protocols for respirometry
- » MiPNet03.02 - Chemicals and media for respirometry
- » MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.
