Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "MiPNet09.12 O2k-Titrations"

From Bioblast
Line 3: Line 3:
|info=[http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions]
|info=[http://www.bioblast.at/index.php/File:MiPNet09.12_O2k-Titrations.pdf Versions]
|authors=OROBOROS
|authors=OROBOROS
|year=2015-02-12
|year=2015-09-28
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=OROBOROS (2014) O2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(11):1.
|abstract=OROBOROS (2015) Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(14): 1-2.


A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.
A list of substrates, uncouplers, and inhibitors used in [[SUIT]] protocols in [[high-resolution respirometry]], with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.

Revision as of 08:41, 29 September 2015

Publications in the MiPMap
O2k-Protocols
Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. »Bioblast pdf«

» Versions

OROBOROS (2015-09-28) Mitochondr Physiol Network

Abstract: OROBOROS (2015) Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(14): 1-2.

A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.

» O2k-Protocols
» Product: OROBOROS O2k, O2k-Catalogue


O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 




Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Inhibitor, Substrate, Uncoupler, Amino acid 


HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-chemicals and media 

Further information

» Excel Templates and DatLab-Demo Files
» MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes.