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MiPNet09.12 O2k-Titrations

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O2k-Protocols
Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations.

Β» Open Access-MiPNet09.12.pdf; Versions

OROBOROS (2014-04) Mitochondr Physiol Network

Abstract: OROBOROS (2014) Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(11): 1.

A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.

>> O2k-Protocols:Overall contents
>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue


β€’ O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 




Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Inhibitor, Uncoupler, Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Amino Acid"Amino Acid" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. 


HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-chemicals and media 

Further information

>> Excel Templates and DatLab-Demo Files

MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes