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MiPNet09.12 O2k-Titrations

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O2k-Protocols
Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations.

Β» Open Access-MiPNet09.12.pdf; Versions

OROBOROS (2014-04) Mitochondr Physiol Network

Abstract: OROBOROS (2014) Oxygraph-2k manual titrations: SUIT protocols with mitochondrial preparations. Mitochondr Physiol Network 09.12(11): 1.

A list of substrates, uncouplers, and inhibitors used in SUIT protocols in high-resolution respirometry, with information on abbreviations, stock xconcentrations, final experimental concentrations, volumes titrated into the 2 ml O2k-chamber, and syringes used for manual titrations.

>> O2k-Protocols:Overall contents
>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue


β€’ O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 




Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Inhibitor, Uncoupler, Substrate;glucose;TCA cycle"Substrate;glucose;TCA cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Amino acid 


HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-chemicals and media 

Further information

>> Excel Templates and DatLab-Demo Files

MiPNet19.14 - SOP for manual O2k-titrations with Hamilton microsyringes