MiPNet10.04 CellRespiration: Difference between revisions
No edit summary |
No edit summary |
||
Line 12: | Line 12: | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Mouse | |organism=Mouse | ||
|tissues=Blood Cell; Suspension Culture | |tissues=Blood Cell; Suspension Culture | ||
|preparations=Intact | |preparations=Intact cells | ||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|kinetics=Inhibitor; Uncoupler | |kinetics=Inhibitor; Uncoupler | ||
|instruments=Oxygraph-2k, TIP2k, Protocol, MiPNet-Publication | |||
|additional=O2k-Demo | |additional=O2k-Demo | ||
|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology |
Revision as of 08:59, 8 August 2013
Garedew A, Haffner B, Huetter E, Gnaiger E. An experiment with high-resolution respirometry: Phosphorylation control in cell respiration. Mitochondr Physiol Network 10.04. |
Β» MiPNet10.04
Garedew A, Haffner B, Huetter E, Gnaiger E (
Abstract: Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with cultured, suspended cells in the OROBOROS Oxygraph-2k (O2k). The experiment demonstrates manual titrations of inhibitors, and automatic titrations of an uncoupler using the electronic Titration-Injection microPump TIP2k. Application of the DatLab 4.3 (upgraded) software is shown for instrumental control (O2k and TIP2k), and on-line data analysis. The following guideline describes the experiment in the form of a laboratory protocol, complementary to the relevant sections of the Oxygraph-2k Manual. The experiments were carried out by participants of an O2k-Course on high-resolution respirometry in April 2005 (IOC30; SchrΓΆcken, Austria).
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels:
Organism: Mouse
Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Intact cells
Coupling state: OXPHOS
HRR: Oxygraph-2k, TIP2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., MiPNet-Publication"MiPNet-Publication" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo