Difference between revisions of "MiPNet12.23 FiberRespiration"
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Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- | Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- | ||
2k (O2k). The experiment demonstrates [http://bioblast.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf manual titrations] applied to study respiratory capacity in permeabilized fibres. Application of the [http://www.oroboros.at/ | 2k (O2k). The experiment demonstrates [http://bioblast.at/images/e/ef/MiPNet09.12_O2k-Titrations.pdf manual titrations] applied to study respiratory capacity in permeabilized fibres. Application of the [http://www.oroboros.at/?datlab DatLab 4 software] is shown for on-line data analysis [[MiPNet12.09]] The following guideline describes the experimental protocol and includes a short discussion of | ||
software] is shown for on-line data analysis [[MiPNet12.09]] The following guideline describes the experimental protocol and includes a short discussion of | |||
results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 [http://www.oroboros.at/index.php?id=ioc44 (IOC44; Schroecken, Austria).] | results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 [http://www.oroboros.at/index.php?id=ioc44 (IOC44; Schroecken, Austria).] | ||
:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | :>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents] | ||
Revision as of 11:06, 29 November 2013
| Lemieux H, Votion DM, Gnaiger E (2007) Mitochondrial respiration in permeabilized fibres: Needle biopsies from horse skeletal muscle. Mitochondr Physiol Network 12.23. |
» Open Access-MiPNet12.23.pdf; Versions
OROBOROS (2007) Mitochondr Physiol Network
Abstract: O2k-Protocol for Mitochondrial Respiration in Permeabilized Fibres: Needle Biopsies from Horse Skeletal Muscle
Methodological and conceptual features of highresolution respirometry are illustrated in an experiment with permeabilized fibres in the OROBOROS Oxygraph- 2k (O2k). The experiment demonstrates manual titrations applied to study respiratory capacity in permeabilized fibres. Application of the DatLab 4 software is shown for on-line data analysis MiPNet12.09 The following guideline describes the experimental protocol and includes a short discussion of results. The experiments was carried out by participants of an O2k-Course on HRR in December 2007 (IOC44; Schroecken, Austria).
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• O2k-Network Lab: AT_Innsbruck_OROBOROS, BE_Liege_Votion DM
Labels: MiParea: Respiration, Instruments;methods
Organism: Horse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Regulation: Coupling efficiency;uncoupling, Cyt c Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
Further information
- >> MiPNet12.23
- >> MiPNet04.07 is integrated in MiPNet12.23.
- Version 1: 2007
- Version 6: 2011-12-11
