MiPNet18.06 Amplex-Yeast: Difference between revisions
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http:// | |title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. | ||
|info=[[Media:MiPNet18.06 Amplex-Yeast.pdf|'''Open Access-MiPNet18.06.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet18.06_Amplex-Yeast.pdf Versions] | |info=[[Media:MiPNet18.06 Amplex-Yeast.pdf|'''Open Access-MiPNet18.06.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet18.06_Amplex-Yeast.pdf Versions] | ||
|authors=OROBOROS | |authors=OROBOROS | ||
|year=2013-08 | |year=2013-08 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''O2k- | |abstract='''Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2013) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06.''' | ||
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2). | Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2). | ||
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | :>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]] | ||
|mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck MitoCom | |mipnetlab=AT Innsbruck OROBOROS, AT Innsbruck MitoCom | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|instruments=Oxygraph-2k, Fluorometry, Protocol | |instruments=Oxygraph-2k, Fluorometry, Protocol | ||
|additional=O2k-Demo | |additional=O2k-Demo | ||
}} | }} | ||
Revision as of 16:55, 17 May 2014
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» Open Access-MiPNet18.06.pdf; Versions
OROBOROS (2013-08) Mitochondr Physiol Network
Abstract: Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2013) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06.
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the O2k-Fluorescence LED2-Module. Extracellular H2O2-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H2O2 production (Fig. 2).
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
• O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Saccharomyces cerevisiae
Preparation: Intact cells
Regulation: O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Uncoupler Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo
