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Difference between revisions of "MiPNet19.03 O2k-cleaning and ISS"

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{{Template:OROBOROS support page name}}
{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Chamber cleaning SOP and Integrated Suction System (ISS).  
|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]] O2k-Chamber cleaning SOP and Integrated Suction System (ISS).
|info=[[File:PDF.jpg|50px|link=http://wiki.oroboros.at/images/d/df/MiPNet19.03_O2k-cleaning_and_ISS.pdf |Bioblast pdf]] |authors=OROBOROS
|info=[[File:PDF.jpg|100px|link=https://www.bioblast.at/images/d/df/MiPNet19.03_O2k-cleaning_and_ISS.pdf |Bioblast pdf]] » [http://www.bioblast.at/index.php/File:MiPNet19.03_O2k-cleaning_and_ISS.pdf Versions]
|year=2015-11-11
|authors=Oroboros
|year=2021-04-27
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Fleischmann S, Gnaiger E (2015) O2k-Chamber cleaning SOP and Integrated Suction System (ISS). Mitochondr Physiol Network 19.03(03):1-4.''' » [http://www.bioblast.at/index.php/File:MiPNet19.03_O2k-cleaning_and_ISS.pdf Versions]
|abstract=Di Marcello M, Komlodi T, Schmitt S, Gnaiger E (2021) O2k-Chamber cleaning SOP and Integrated Suction System (ISS). Mitochondr Physiol Network 19.03(07):1-8.  
:>> Product: [[Oxygraph-2k|O2k]], [[OROBOROS O2k-Catalogue | O2k-Catalogue]]
[[Image:MitoPedia.jpg|left|60px|link=http://www.bioblast.at/index.php/MitoPedia: DatLab|MitoPedia: DatLab]] [[O2k-chamber]]
|mipnetlab=AT_Innsbruck_OROBOROS
{{MiPNet pdf page linking to MitoPedia}}
:» Product: [[ISS-Integrated Suction System]]
|mipnetlab=AT_Innsbruck_Oroboros
}}
}}
{{Labeling
|area=Instruments;methods
|instruments=Oxygraph-2k, O2k-Protocol
|additional=O2k-SOP
}}
== Supplement ==
A properly assembled chamber can remain in the oxygraph for a long time. However to avoid the chamber getting stuck due to dried media and to detect damages or contaminations we recommend to disassemble, clean and  reassemble the chamber at least every 1 - 2 years.


=== General cleaning instructions ===
== Additional resources ==
» [https://wiki.oroboros.at/index.php/Talk:MiPNet19.03_O2k-cleaning_and_ISS#Problem_solved Chemicals contamination]


There are at least three fundamentally different kind of contaminations that can accumulate in the measuring chamber of the oxygraph and cause problems. All of them have to be treated in different ways:
* [[Biological contamination]]: The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). If this does not help the biological contamination may be embedded in e.g. protein contamination and the glass chamber has to be disassembled and cleaned as described below:
* Protein contamination and other macroscopic deposits. After long use a whitish deposit can form at the glass walls of the chamber. Additionally, small glass splinters (difficult to see) may become embedded in such a deposit. This will be detected by a jumping stirring bar or a stirring bar getting stuck. In this case the glass chamber should be removed from the oxygraph and treated with concentrated hydrochloric acid at least over night. Use a stirring plate and the O2k stirring bar, in this way the stirring bar  is cleaned simultaneously.
* [[MiPNet19.03_O2k-cleaning_and_ISS|Contamination by hydrophobic inhibitors]]: The ideal counter agent for this case is pure ethanol.
* '''Cleaning with cells, tissue or isolated mitochondria''': If there are indications of carry-over of inhibitors or uncouplers, it is recommended to wash the chambers with cells, tissue or isolated mitochondria stored at -20°C. Inhibitors will be bound to the mitochondria when filling the chamber up to the rim such a suspension. If live cells are available, these are preferred due to their superior binding capacity. The suspension should be left in the chambers with inserted stoppers for at least 30 min followed by cleaning the chambers according to the SOPs.


== Keywords ==
{{Template:Keywords: Chamber volume}}
{{Template:Keywords: Oxygen signal}}


[[Talk:Cleaning the glass chamber|Further information >>]]


{{Labeling
=== Unintentional introduction of inhibitors to the O2k-Chamber ===
|area=Instruments;methods
 
|instruments=Oxygraph-2k, O2k-Manual
Inhibitors may also be introduced unintentionally, one example being 70% ethanol used in hospital settings containing antiseptics. Such inhibitors may accumulate e.g. in plastic parts and inhibit subsequent experiments. See the [[Talk:MiPNet19.03_O2k-cleaning_and_ISS#Apparent_carry-over_of_CI-inhibitors_versus_contamination_in_chemicals_.28update:_solved.29| discussion page]].
|additional=O2k-SOP
 
}}
== Further details ==
:>> [[O2k-Chamber]]
:>> [[MiPNet12.08 O2k-Calibration]]
:>> [[MiPNet14.06 InstrumentalO2Background]]

Revision as of 14:51, 27 April 2021


                  


O2k-Open Support

MiPNet19.03 O2k-cleaning and ISS


Publications in the MiPMap
O2k-Manual
O2k-Chamber cleaning SOP and Integrated Suction System (ISS).

» Bioblast pdf » Versions

Oroboros (2021-04-27) Mitochondr Physiol Network

Abstract: Di Marcello M, Komlodi T, Schmitt S, Gnaiger E (2021) O2k-Chamber cleaning SOP and Integrated Suction System (ISS). Mitochondr Physiol Network 19.03(07):1-8.

MitoPedia: DatLab

O2k-chamber


O2k-technical support and open innovation
Open the pdf document above.
» Current O2k-series: NextGen-O2k Series XB and O2k Series J
» Current software versions DatLab 8.0: MitoPedia: DatLab
» Product: ISS-Integrated Suction System


O2k-Network Lab: AT_Innsbruck_Oroboros


Additional resources

» Chemicals contamination


Keywords


Questions.jpg


Click to expand or collaps



Questions.jpg


Click to expand or collaps



Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, O2k-Manual 

O2k-SOP