Difference between revisions of "MiPNet20.13 Safranin mt-membranepotential"
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|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01): 1-5.''' | |abstract='''Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01): 1-5.''' | ||
|mipnetlab=AT_Innsbruck_OROBOROS | |mipnetlab=AT_Innsbruck_OROBOROS | ||
}} | }} | ||
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|instruments=Oxygraph-2k, Fluorometry, Protocol | |instruments=Oxygraph-2k, Fluorometry, Protocol | ||
}} | }} | ||
== Reference == | |||
* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. Β»[[Krumschnabel_2014_Methods_Enzymol| PMID: 24862266]] | |||
Β | * [[Safranin]] used in the experiments for this publication was Safranin O from Sigma (# S2255, 25 g). | ||
Β | |||
Revision as of 11:17, 13 December 2014
Safranin data acquisition and analysis (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. |
Β» Open Access-MiPNet19.19.pdf; Versions
OROBOROS (2014-10) Mitochondr Physiol Network
Abstract: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01): 1-5.
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Nervous system
Preparation: Homogenate
Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Reference
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. Β» PMID: 24862266
- Safranin used in the experiments for this publication was Safranin O from Sigma (# S2255, 25 g).