Difference between revisions of "MiPNet20.13 Safranin mt-membranepotential"
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|additional=O2k-Demo, O2k-MultiSensor | |additional=O2k-Demo, O2k-MultiSensor | ||
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:» The OROBOROS MitoLab uses [[Safranin]] O from Sigma (#S2255, 25 g). | |||
:» More details: [[Talk:MiPNet19.19 Safranin DataAcquisitionAnalysis |»Discussion«]]. | |||
== Reference == | == Reference == | ||
* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. »[[Krumschnabel_2014_Methods_Enzymol| PMID: 24862266]] | * Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. »[[Krumschnabel_2014_Methods_Enzymol |PMID: 24862266]] | ||
Revision as of 12:31, 6 June 2015
Safranin data acquisition and analysis. O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. »Bioblast pdf« |
» Versions
OROBOROS (2015-05-27) Mitochondr Physiol Network
Abstract: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2015) O2k-Fluorometry: HRR and simultaneous determination of mt-membrane potential with safranin. Mitochondr Physiol Network 19.19(01):1-5.
• O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Nervous system
Preparation: Homogenate
Coupling state: LEAK, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Fluorometer, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-Demo, O2k-MultiSensor
- » The OROBOROS MitoLab uses Safranin O from Sigma (#S2255, 25 g).
- » More details: »Discussion«.
Reference
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-81. »PMID: 24862266