Difference between revisions of "MiPNet25.03 IOC144 Innsbruck AT"

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|  [[Hussein Rawya]]
 
|  [[Hussein Rawya]]
 
| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
 
| '''[[SE Lund Elmer E]]''' - Lund University, Lund (SE)
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|-
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| [[File:Profile-icon-9.png|70px|Participant]]
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|  [[Linzi Jin]]
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| '''[[CN Beijing Huawei]]''' - Huawei, Beijing (CN)
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| [[File:Profile-icon-9.png|70px|Participant]]
 
| [[File:Profile-icon-9.png|70px|Participant]]

Revision as of 13:29, 4 December 2019

Oroboros Instruments

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MiPNet25.03 IOC144 Innsbruck AT


Publications in the MiPMap
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Innsbruck AT, 2020 Jan 20-22. O2k-Coaching Days - Basic IOC144.

»

Oroboros (2020-01-20) Mitochondr Physiol Network

Abstract:
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O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.


O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
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General information

The course is fully booked. In case of cancellations further registrations can be accepted. In order to be added to the waiting list, send an email to maximilian.reinelt@oroboros.at
O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.


Venue

» Oroboros O2k-Laboratory
Schoepfstrasse 18, 6020 Innsbruck
» Information on travel and venue: IOC Innsbruck.
» Hotel recommendation: Basic Hotel Innsbruck***(https://www.basic-hotel.at/en/)
Using code "OROBOROS" for booking online you will get the Single Room for € 85.00 and the Double Room for € 120.00 including breakfast. Rooms are available at a first come first served basis.
Free cancellation until 7 days prior. Booking requires a credit card for guarantee. Payment on site. A 2 € City tax to be charged additionally.

Registration


Registration closed.


Tutors and lecturers


Preliminary programme


Monday 20th

Topic Room
08:15 Welcome - Get-together: Introduction of participants and their research interests Oroboros O2k-Laboratory
08:30 OroboPOS service and O2k instrumental setup - overview with videoclips Oroboros O2k-Laboratory
09:15 Hands-on: OroboPOS service and O2k instrumental setup Oroboros O2k-Laboratory
09:55 DatLab 7.4 overview Oroboros O2k-Laboratory
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration Oroboros O2k-Laboratory
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips Oroboros O2k-Laboratory
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

Oroboros O2k-Laboratory
15:00 Coffee
15:30-17:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. Oroboros O2k-Laboratory


Tuesday 21st

Topic Room
08:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
09:15 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 Oroboros O2k Laboratory
09:45 SUITbrowser: how to find the best SUIT protocol for your research questions. Oroboros O2k Laboratory
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).

DL-Protocol: SUIT-001 O2 ce-pce D003.DLP

DL-Protocol: SUIT-002 O2 ce-pce D007.DLP

Oroboros O2k Laboratory
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use (continuation)

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
13:30 DatLab 7.4 analysis: Introduction and new features Oroboros O2k-Laboratory
15:00 Coffee
15:30-17:30 Individual DatLab analysis + summary Oroboros O2k-Laboratory


Wednesday 22nd

Topic Room
08:30 MitoFit proficiency test

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
10:00 Coffee
10:30 MitoFit proficiency test

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).

DL-Protocol: SUIT-001 O2 ce-pce D003.DLP

DL-Protocol: SUIT-002 O2 ce-pce D007.DLP

Oroboros O2k-Laboratory
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use (continuation)

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
13:15 DatLab analysis Oroboros O2k-Laboratory
14:00 O2k-Applications - overview Oroboros O2k-Laboratory
14:30 Proficiency test: DatLab analysis summary Oroboros O2k-Laboratory
15:00 Coffee
15:30 The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE MiPArt
16:30 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt

Participants

Participant Institution
Participant Buoso Chiara AT Innsbruck Weiss G - Medical University Innsbruck, Innsbruck (AT)
Participant Beleza Jorge PT Porto Beleza J - University of Porto, Porto (PT)
Participant Carter Kristen US MS Jackson Cornelius DC - University of Mississippi, Jackson (US)
Chamkha Imen Chamkha Imen SE Lund Elmer E - Lund University, Lund (SE)
Participant Coldewey Sina DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Participant Esper Treml Ricardo DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Participant Gayathri Narayanappa IN Bangalore Gayathri N - NIMHANS, Bangalore (IN)
Kate Griffiths Griffiths Kate UK Glasgow Selman C - Glasgow University, Glasgow (UK)
Participant Hussein Rawya SE Lund Elmer E - Lund University, Lund (SE)
Participant Linzi Jin CN Beijing Huawei - Huawei, Beijing (CN)
Participant Neu Charles DE Jena Coldewey S - Jena University Hospital, Jena (DE)
Rita Pereira Pereira Rita PT Porto Beleza J - University of Porto, Porto (PT)
Participant Place Nicolas CH Lausanne Place N - Université de Lausanne, Lausanne (CH)
Participant Participant SE Lund Elmer E - Lund University, Lund (SE)

Contact

Oroboros Instruments GmbH
High-Resolution Respirometry
Schoepfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
E-Mail: instruments@oroboros.at
Mitochondria and Cell Research


IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.


Labels:






ORO, IOC, MitoGlobal, Next, 2020