MitoKit-CII/Malonate-nv: Difference between revisions

Revision as of 15:10, 11 April 2019

high-resolution terminology - matching measurements at high-resolution

MitoKit-CII/Malonate-nv

Description

NV161/MitoKit-CII (diacetoxymethyl malonate) is a plasma membrane-permeable (p) prodrug that diffuses across the plasma membrane. Cleavage of diacetoxymethyl residues is mediated by intracellular esterases, thus releasing malonate in the intracellular space.

Abbreviation: Mnanv

Reference: MitoKit-CII, MitoKit-CII MitoPedia, Ehinger 2016 Nat Commun

MitoPedia topics: Media for respirometry

Application in HRR

Mnanv: NV161 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol^-1

Preparation of 100 mM stock solution:

Add 395.78 µL DMSO in 7.7 µL NV161.
Divide into 40 µL portions.
Store at -20 °C.

Preparation of 10 mM stock solution (from the 100 mM stock):

Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
Divide into 100 µL portions.
Store at -20 °C.

» O2k manual titrations

Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
Final concentration: 5 µM steps
Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.

@@ Line 7: / Line 7: @@
 |mitopedia topic=Medium
 }}
-* For preparation of MitoFit-CII, see [[MitoKit-CII MitoPedia]].
+== Application in HRR ==
+:::: '''Mnanv: NV161''' 10 mg, 7.7 µL, 5.24 M, MW=248.1 g.mol<sup>-1</sup>
+:::: '''Preparation of 100 mM stock solution:'''
+::::# Add 395.78 µL DMSO in 7.7 µL NV161.
+::::# Divide into 40 µL portions.
+::::# Store at -20 °C.
+:::: '''Preparation of 10 mM stock solution (from the 100 mM stock):'''
+::::#	Dilute 40 µL 100 mM stock solution with 360 µL DMSO.
+::::#	Divide into 100 µL portions.
+::::#	Store at -20 °C.
+:::» '''O2k manual titrations'''
+::::* Titration volume: 1 µL steps using a 10 µL syringe (2 mL O2k-Chamber).
+::::* Final concentration: 5 µM steps
+::::* Please consider that the final concentration needed in the O2k-Chamber may vary according to your cell type.