MitoPediaAlert2020 Archive: Difference between revisions

Revision as of 12:27, 30 April 2020

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

Apr 2020

May-07 Th

Do you know how to evaluate fatty acid oxidation (FAO, F-pathway) avoiding to overestimate it?

Did you know that malate alone might support mt-respiration in mitochondrial preparations, if malate anaplerotic pathways (e.g. mt-malic enzyme) are present?

- Learn more about - »malate anaplerotic pathway control state«

- DatLab 7.4 provides a step-by-step guide through the SUIT-027 to evaluate the malate anaplerotic pathway - »SUIT-027«

- Since F-pathway can be overestimated due to anaplerotic reactions, SUIT-002 was specially designed to give information on F-pathway in OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways - »SUIT-002«

Apr-24 Fr

ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the electron transfer system.

- »Hatefi 1962 J Biol Chem-XLII«

Apr-21 Tu

Citrate synthase activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.

- »MiPNet17.04 CitrateSynthase«

Apr-13 Mo

Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?

- DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation - »SUIT-024«

Apr-08 We

The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.

- DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration. - »SUIT-010«

Mar 2020

Mar-16 Mo

The standardized coupling control protocol to explore the bioenergetics of living cells

- DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »SUIT-003«

Mar-11 We

Does blebbistatin reduce oxygen dependence of respiration of permeabilized muscle fibers? This is one of the questions addressed in the COST Action CA15203 MitoEAGLE WG2.

- »Blebbistatin«

Mar-06 Fr

Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols.

- »RP1« and »RP2«

Mar-03 Tu

Rapamycin - more differences or more in common with other mitObesity drugs?

- »Rapamycin«

Feb 2020

Feb-26 We

It is essential to titrate uncouplers in multiple steps for respirometric measurement of electron transfer capacity.

- »Noncoupled respiration«

Feb-24 Mo

More research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«

Feb-19 We

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«

Feb-18 Tu

Why do we fight gas bubbles?

- Dive deep into »Oxygen solubility«

Feb-12 We

What do metformin, melatonin and other bioactive mitObesity compounds have in common?

- »MitObesity drugs«

Jan 2020

Jan-16 Th

Oxygen flux

- which SI units should be used?

Did you ever think that units for body mass could be an issue?

- »Body mass«

Jan-08 We

Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.

@@ Line 2: / Line 2: @@
 </big>__TOC__
 == Apr 2020 ==
+* '''May-07 Th'''
+::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''
+:::: Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present?
+:::::::- Learn more about - »'''[[Malate anaplerotic pathway control state|malate anaplerotic pathway control state]]'''«
+:::::::- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«
+:::::::- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«
+----
+* '''Apr-24 Fr'''
+[[File:ETC-.JPG|right|150px|Electron transport chain]]
+:{|
+| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
+| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer-pathway |electron transfer system]].
+- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«
+|}
+<br />
+----
+* '''Apr-21 Tu'''
+[[File:CS.png|right|100px|Citrate synthase]]
+:{|
+| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]
+| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.
+- »'''[[MiPNet17.04 CitrateSynthase]]'''«
+|}
+<br />
+----
 * '''Apr-13 Mo'''
 :{|